It's a video from 2013 but I just found it and want to thank you. This really helped me understand how the reactions work between Hb, O2, CO2 and H+. And also how allosteric regulator works for Hemoglobin.
Man idk what you did with your life but I hope you accomplished great things. You're and awesome tutor. I would pay you the money I give to my shitty class for some lectures like this. Thanks
Hi you referred to a video before this one. Which video on Hemoglobin is that? Talking about the cooperativity. Thank you so much!!! This was so helpful!
The Histidine since it's found the myoglobin being positively charged amino acid but why saying it's the T- State of Hemoglobin conformation being stabilized . Kindly make mention of the connection between these two globular proteins Sir .
•2,3BPG lowers the affinity of Hb for oxygen (raises the P50 of Hb from ~12 torr in aqueous solution to ~26 torr in blood - i.e. respiring tissue) Can you explain the info in parentheses? Its on my professors slides, how does this change the P50?
3wJblind0114 Yeah. The p50 of Hemoglobin (Hb) is the partial pressure of oxygen necessary to make Hb 50% saturated. 2,3-BPG (being an inhibitor of Hb) raises the p50. That means, with an inhibitor, Hb requires more oxygen (26 torr) to be 50% saturated, whereas without an inhibitor, Hb only requires 12 torr of pressure due to oxygen to reach 50% saturation. I apologize if this response is two weeks too late, though. :/
pls mention in the video that what ever u said all is written it took me week to write down what ever u said and then after a 1week what i saw was ..... pls mention it
King James Details aside, people who've grown accustomed to living at high altitudes (where O2 concentration/pressure is lower, essentially) have higher 2,3-BPG levels. The higher 2,3-BPG levels causes the oxy-Hb to more readily give up is O2 to the tissues. This way, the tissues are not O2-deprived, despite the lower O2 levels at higher altitudes. This is the basis for altitude training. Distance athletes will often train at higher altitudes over long periods of time so as to get their bodies adjusted to the lower O2 concentrations/pressures. This way, their bodies basically get better at getting O2 delivered to their tissues, helping to increase their performance during races, particularly races at lower altitudes.
+verdeapplesify I think it's because 2,3-BPG binds in the tissues, which is at a much lower pH due to CO2 from the muscles. The CO2 from the muscles is turned to carbonic acid. The extra protons flying around bind to histidine, which gives the it a positive charge, allowing the 2,3-BPG to bind. At least that's how I understood it!
