Hierarchical Clustering: DENDROGRAMS - what are they, and how are they used? 

Happy to hear the video helped :)

Glad it was helpful!

I'm glad you liked it. I appreciate the feedback. Thanks!

I appreciate your feedback. I'll make a note to make a video about identifying the optimal number of clusters - thanks for the suggestion.

Funny you should ask that ... the following paper is next on my reading list: "Pulsed-field gel electrophoresis (PFGE) analysis of Listeria monocytogenes isolates from different sources and geographical origins and representative of the twelve serovars" www.academia.edu/111312006/Pulsed_field_gel_electrophoresis_PFGE_analysis_of_Listeria_monocytogenes_isolates_from_different_sources_and_geographical_origins_and_representative_of_the_twelve_serovars

I've looked at this in a bit more detail, and to be honest, handling these type of data is beyond my area of expertise. I did find some general information that I found helpful: A guide to interpreting electrophoresis gels: bento.bio/resources/bento-lab-advice/interpreting-electrophoresis-gels-with-bento-lab/#:~:text=The%20smallest%20bands%20are%20at,is%20up%20the%20ladder%20scale. (pulsed-field addressed larger DNA molecules but I presume the principles on interpretation remain the same). Any analytical technique requires digital data. I found this: Data processing of pulsed-field gel electrophoresis images www.ncbi.nlm.nih.gov/pmc/articles/PMC6940661/ The data processing would seem to me the critical step, which will ultimately result in the generation of tabulated data that would be amenable to cluster analysis. The columns of this tabulation would correspond to metrics that describe the banding, which each sample being represented by a row. I would guess that this data processing is integrated into most laboratory systems that produce pulsed-field electrophoresis gel?

Thank you so much!