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Cleaner Immunoprecipitation, Reduced Sample Requirement, and Simplified Sample Preparation for Ubiquitination and SUMOylation
Featured Speakers: Alissa Nelson, PhD, (Sr. Proteomics Scientist, Cell Signaling Technology) and Jeroen Demmers, PhD, (Dir. Proteomics Core Center & Assoc. Professor, Erasmus University Medical Center)
Watch this webinar recording to learn:
● How to enrich PTM peptides for LC-MS analysis;
● The importance of ubiquitination and SUMOylation;
● How PTMScan HS differs from PTMScan "classic," both in method and results;
● How PTMScan HS performs in a research lab environment.
Contents:
0:00 Introduction
3:27 CST® PTMScan Technology
4:20 The PTMScan Method
5:30 Proteomics services at CST
5:52 Characterization of ubiquitinylation sites by PTMScan
6:50 SUMOylation site characterization
7:38 PTMScan HS (High specificity, sensitivity, simplicity)
10:50 Comparison of Urea In-Solution Digestion and S-traps
14:40 Comparison of PTMScan HS and Classic
16:58 High Sensitivity and Specificity for SUMO
18:35 Summary
21:03 Protein ubiquitination dynamics: Introduction
22:26 The ubiquitin protease system (UPS)
24:33 Inactivation of the proteasome
28:44 Ubiquitinome: diGly peptides are highly upregulated
31:48 Functionally different polyubiquitination types
33:37 Characterization of three proteasome associated DUBs
35:13 Improved detection of diGly peptides
38:05 TMT labelling of diGly peptides
42:57 Targets of USP7
49:22 Q&A
About CST: Cell Signaling Technology (CST) is a private, family-owned company, founded by scientists and dedicated to providing high-quality research tools to the biomedical research community. Our employees operate worldwide from our U.S. headquarters in Massachusetts, and our offices in the Netherlands, China, and Japan. cellsignal.com/about
Cell Signaling Technology, CST, and PTMScan are registered trademarks of Cell Signaling Technology, Inc. All other trademarks are the property of their respective owners. cellsignal.com/trademarks
#proteomics #antibody
17 июл 2024
