How a drop of pond water looks under the microscope 🔬 241 

Much easier! I did a color adjustment in the video editing program. But I also have a DIC microscope, which also helps.

@@Microbehunter so cool thx 4 the reply mate. U do great work for us all. Could i unscrew say the 40x objective and screw in a rad DIC 40x one? Would be a nice upgrade.

This (unfortunately) does not work. DIC requires not only special objectives, but also prisms, filters etc. etc. and only high-end microscopes can be upgraded to do this. And it is expensive. However, there is a very attractive alternative, called Oblique Illumination, which also produces the 3D effect by creating shadows, similar to DIC. You might also want to experiment with Rheinberg and Darkfield. Video here: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-Pku4T8s-0lg.html

@@Microbehunter Thx for the nice answer mate. Yes i saw this vid and use the 3d filter sometimes. I likes your color mainly mate, great detail and quite impressive against others. I too will try n enhance my 14MP camera in the setting if i have them. I use my compound microscope for my medical cannabis growth/ dev, IPM, soil & runoff microbes. Your welcome to interview me for rad uses of the microscopes mate, i'm a post uni research med can student. Keep up your great work amigo!

Color adjustment in the video editor made the background blue.

This is a water planet my freind.

Yes I can do that, but maybe it will not be very interesting... But I can try.

@@Microbehunter thanks

I changed the color in the video editor (DaVinci Resolve). Original color is gray.

DIC and a few clips in Phase

@@Microbehunter thanks for this insteresting video and comments, which model of microscope did you use for the DIC images ? i love how DIC images turn out, do you know which model is the cheapest or has the best ratio price/ quality ? thank for your help!

@@elyrose3480 If I’m not wrong he uses an Olympus BX53 with DIC

@@darryllim2969 thank you for your reply. I whish it was less expensive :(

Full HD video (1080p) has about 2 megapixel. I use 4K, which has about 8 Megapixels. This is more than the microscope can deliver and the 4K resolution is much higher than what a normal monitor is able to display anyway (and youtube videos are shown small). So it is not a resolution issue alone. the reason why the images appear so clear is because of several reasons: First I have a DIC microscope, which makes the specimen appear to cast a shadow, and increases contrast. So the edges are more visible and you get the subjective impression that the image is clear. Second, I do a lot of editing of the original footage. I use the free video editor daVinci Resolve (can be downloaded), which allows me to adjust pretty much anything. The software is powerful but complex, however. The camera also captures a very large field of view, so there is very little "empty" magnification. So there are several factors that play a role. You will be surprised what simple contrast enhancement is able to do to improve the image quality. While no image processing technique is able to do miracles, I would first do a contrast enhancement (levels adjustment).

When the camera only takes an image from the center of the field of view (and not the whole), then this image is magnified "artificially". It will appear blurry. It is magnification without additional information (beyond the resolution of the microscope) and this is then called "empty magnification". So this is magnification that does not add "value". You can also get empty magnification by using very strong eyepieces. It will appear larger but more blurry. In case of mobile phone cameras, they catch the full field of view, but the image will appear in a circle and you lose pixels this way. You have to crop out a rectangle inside the circle and therefore you again lose some image information.

@@Microbehunter: I have seen this in practice. Thank you for the reply. I do like to keep the circle or at least enough of it to see the diameter of the field of view as seen through the microscope lenses. I almost always use my 10x eyepieces. Putting that circle up on a laptop screen allows me to roughly calculate the size of the specimen by measuring it in mm in relation to the diameter of the field of view, then converting those sizes to microns. If I crop to a rectangle, like you said, I lose more pixels and also any chance of getting measurements of microbes or blood cells accurate. Measuring sizes of specimen is one of my priorities and indeed pleasures. If I keep the circle, it doesn't matter how many times it is zoomed or magnified, the scale of everything within the circle is constant. On video, I usually zoom in to around 2.5 times on my phone camera, this leaves the edges of the circle visible enough to take a measurement across the diameter on the computer screen. So far, I have been very happy with my still images but always looking for ways to make improvements, as there is always room for improvement. I do have a video software program and it can adjust contrast and sharpness, but it is not anywhere near as complex as your software is. My phone camera actually has 13mps not 5 as I stated in my previous comment.

Paramecia are found in ponds and streams. You can use a small banana peel to feed them, but they do not live on bananas.

@@Microbehunter Ok

put some solid stuff from the bottom of the pond etc. on the slide, not only water.

@@Microbehunter thank you for your quick response we just found your channel and are looking forward to learning more