How I Turn a 10cc Spore Syringe into 310cc's of Mycelium Liquid Culture to Grow Mushrooms at Home 

Nice tutorial i like the recipe, just a few comments on your other processes. Why did you inject an entire 10cc spore syringe its a waste of spores you could have added a few drops, and did you qc the syringe on agar before to make sure its not contaminated? Also at the end you ruin the sterility of your LC when you add it to the syringe in a few ways. Firstly, assuming you autoclaved the LC syringe, you negated that by placing it on non sterile surfaces, then spraying it with a sanitizer(spraying iso on a sterile object makes it sanitary and no longer sterile, 2 very different things). Second when you pull the sanitizer into the syringe you make the inside of the syringe no longer sterile. Lastly when you run the needle through the micropore tape you expose a large hole to open air then put your fingers all over it. Not trying to hate or anything a lot of people dont have the air quality to work like this without contaminations is all. Here's what I'd do differently: prep: Autoclave the LC syringe in tin foil and keep it wrapped until ready. Use a RTV injection port (you can stab those thick blunt tip LC needles thru it many times) process: Operating in a flow hood or still air box. Prepare by exposing the connection end of the syringe and place it down not touching any surfaces. Open the syringe and slide it half way out of the package, remove cap inside sterile package. Remove syringe completely and attach it to sterile needle by holding the needle from the tin foil making sure never to touch it. You can flame sterilize the needle but its not necessary if you pressure cooked it well. Wipe and spray 70% iso on RTV port. Put syringe in RTV injection port and suck up ur LC. When you finish pull the needle out(the jar remains sterile). Remove needle, you can touch it now. Put syringe in sterile package and secure the cap inside of it without ever touching it.

Psychedelics are just an amazing discovery. It's quite fascinating how effective they are for depression and stress..saved my life.

Psilocybin mushrooms are just filled with so many health benefits. Psilocybin mushrooms treatment saved my life from depression and excessive alcohol use. Never thought I would be saying this about mushrooms.

3:00 Candymaker has a tip for you: Sugar starts to crystalize if you only use one type of sugar with water and heat - no matter which sugar you use (glucose, sucrose, fructose). Corn syrup contains only glucose sugar (usually something like 30% of weight is glucose while the other is water). If you mix glucose with sucrose (in your case something like 1-2g sucrose into the 12g syrup as around 3-4g of the syrup is glucose), it will not crystalize and you have not to worry about it.

I got a 10cc syringe from a reputable dealer. I have used a grand total of 4cc from the syringe and in return I have 10 flourishing, clean agar plates and 3 quarts of popcorn inoculated. The remaining 6cc in the syringe is so dense with spores it's amazing. The agar plates are for isolating strong specimens, then the LC comes from my favorite from that. For a $21 syringe, you can do a WHOLE LOT. For sheer ghetto points, I have never even screwed the provided syringe on. I have used 4cc of spores completely bareback from the lurelock port. I observe reasonable aseptic techniques but do not have a laminar flow system. None of the 10 agar plates are contaminated.

Easiest, most straightforward video ive seen yet.🙏🏻

LME liquid malt extract will show growth in a couple of days, i used to use karo but LME is a game changer!

You are a rare breed on RU-vid, sir. Your videos are direct, not gimmicky, easy to follow, and delivered in a professional manner. Thank you for your efforts. One question. What gauge is the blunt 4 inch needle?

Give yourself another gift: If you tab the tape on the roll when you're done, it makes it easier to pull for next time, plus you already have your tab ready to go! Great videos and love your calm energy, thank you!

Thank you Jared for the All in one grow kit. Blessings to you and your family❤

What a great pedagogue you are. Can't stop watching your videos. Little question here. Where (i guess fridge) and how long does your liquid culture keep?

If u add honey or kyro syrup and place a heating pad near it u could have tripled that growth and if u used that spore syringe and did 3 different jar tripled the amount as well. Still a great video tho!

So helpful for a noob like me. You’re the man!

As I am just starting out in this endeavor your video simplifies what I had always imagined could and is being done- thank you!

Brother, your videos are fantastic, informative and straight to the point! Thank you for all you do! ❤

Will the alcohol kill/ruin the spores if it doesn’t dry completely? I noticed when you sprayed the top before drawing the spores up it was very wet still and again when you sprayed the stopper you put on until you use a needle, do you wait for the alcohol to dry normally?

Very interesting. I'm just starting to learn all this stuff and that was very helpful. Thank you.

Your channel is the best on RU-vid. We love you!

It takes 1 to 3 weeks for lc to colonize. Thanks for the updated steps!

Thanks. I had given up on trying to cultivate at home until watching this.

Just wanted to add on the contamination worries especially for us first rate newbies,.. if its too overwhelming to make mason jar blenders and opening jars etc,.. make the liquid substrate jar with an injection port,.. and inject bought liquid syringes. This is not to knock the poster’s skills,.. i just find this video very advanced compared to where i am at this point in time. Also i live in a 200 year old house from hell (building a new place thankfully) but theres every possible way to contaminate in this house i am in right now. It would increase my chances of success if i bought a pre done spring and injected it into a sterile jar of liquid culture. I can learn to make that jar, to add nutrient and inject sterile air etc. but making the Petri dish, cutting it etc thats a ton of opportunity to add contaminants when done by me😊.

This is the only video I've watched like this! Thank you!!

Really great hair day for sure bro. That's really all that matters in life

Nice work! Getting ready to do some of these myself and the information is very appreciated.

Thank you for reporting in grams and millimeters. :)

You seem pretty chill. Going to check out more of your videos.

What are the secret goodies you add before pressure cooking? Nutrients? Sugars?

My question is, if in 6 months I take one of the syringes and repeat the process, will I ever need to buy any of that strain ever again? Should be repeatable forever, correct?

Thank u so much this looks so much simpler than I had anticipated

@4:35 - that is the most beautiful Snow Globe ever :)

What are the "nutrients" u inject in jar prior to pressure cooker process?

Thank you friend! This is important information!!!!!!💚 "Thank you for watching." Thank you for making!!!!!!!!!!!!!lol

SWIM copied your tek, did not use a measuring device, used 5 inch dispensing needs as well. Karo syrup; but did follow what someone below mentioned in that 10cc was a waste, so SWIM uesd anywhere from 2-4cc depending if the syringe was overfilled or not for 4 different jars , 4 different strains. Took about 5 days to see some mycelium growth, but its going. SWIM did however go with 1-2cc for my next batch and those are about 2 days later and they have all colonized.

Folding the tape is called a buddy tag.

If I only have 1cc of liquid culture in a syringe will that be enough to start a new jar of liquid culture?

Great video. Thank you for your information. So now you have 10 30cc syringe where you drew mycelium (not spores in through a 12ga needle. When you are ready to dispense into spawn jars or bag,, do you have to use same sized 12ga needle? Or a more standard sized injection needle?

You made it look so easy!!

bro thats easy af, thank you!

do instant pots work in place of a standard pressure cooker?

Thanks for sharing this concise and informative coni👍🏼

I don't have a pressure cooker. Can I use a rice cooker?

Thank you, using your website. Enjoyed the video, like your style.

I loved the proprietary joke 😂😂😂

You crack me the hell up, love you broski.❤

Great video 🤘

Did you use a cabinet against air germs?

Can you transfer a grow bag with mycelium to a monobox with substrate? Ty

@1:44, what nutrients?

What kinda nutrients do you use in that first syringe?

When you first suck up ethanol with the syringe and then the mycelium, you end up having the whole needle full of ethanol which will definitely kill your mycelium if you don't suck up as much as you do in this video (for example only 1 ml) .... I don't know much about mushroom growing, but I am veterinarian so I have a bit of a clue of how to work sterile, and when you want to make your needle sterile WITHOUT killing the thing you are pumping through it, you either sterilize it in an autoclave or you simply flame it for a second with a lighter.

can i use honey? in place of corn syrup thanks and keep up the good work

The alcohol not being dry before injection doesn’t kill the spores? How?

THIS IS ONE THE BEST CHANNEL I HAVE COME IN CONTACT , PLEASE TELL US THE NUTRIENT YOUR USING . IS IT LME PDA SYRUP HONEY PEPTONE??? REPLY BEFORE I MAKE MISTAKE THANKS AND REAMEN BLESSED

Cool video bro, live culture rocks!

One could add much less water to create a more concentrated spore/mycelium solution, maybe a 100mL. So as to not add so much liquid solution to the grain.

Hey brother Hope you are well Just wanted to tell you that your channel inspired me a lot I'm also trying to start my own channel now growing cannabis and mushies Mad luv Peace Mad scientist

Can you use jelly powder(no gelatin) instead of agar powder in Petridish? Pls reply fast

In order to get mycelium growing the quickest… is it faster to turn spore syringes into LC rather than just inoculating my grain with the spore syringe?

Hey brother Love your channel Thanx for all the information you provide Also busy with my own cannabis and mushies channel Mad love bro Peace

Thank you for sharing. off to have a mooch on your site..

Can you start this expansion with a syringe of liquid culture rather than a spore syringe?

I need some spores where can i get some //??

Very helpful! Thanks!

Hahaha 😂 dude is WRECK’T! I love it

At 0:33 ... I definetely am cultivating the whatever type of mushrooms if you know what I mean. 😅

How long can it stay in the jar before putting in syringes

If I want to use peptone and malt extract how much of each do i use?

Thanks for the rad work!

So if I already have a 10cc syringe of LC, how would I multiply that. Would I follow the same steps, just instead of 10cc of MS use 10cc of LC?

I really hope you see this. Do you have to use a pressure cooker or are there any low and slow methods that would do the same?

Ya man, you look fabulous! grin.

Thank you so much, great video

Awesome video! Thank you for the lesson!

I have about 4 mls left in a syringe I purchased tobtry making grain spawn cud I try this with what's left?

Wait what’s in the “nutrients”? Corn syrup?

I’m confused. Did you not chance contamination with the spoon and syringe by pulling liquid into and out of that same syringe when you added nutrients ? Or was that off camera ?

Awesome video dude! Question, I want to do this but syringe is a live culture syringe with mycelium in it instead of spores. Can I do the same process and just inject the jar with the mycelium I already have instead of spores?

+1 subscriber my man! good work

Hi there, I have a question: Is there any other sweet nutrient to replace the corn syrup or does the nutrient have to be exactly corn syrup? Thanks for your advice!

GOOD LUCK TO YOUR WORK SIR, AM ABOUT TO START NOW , THE NUTRIENT YOUR USING IS IT LMA OR AGGA AGA. NUTRIENT AGGA ? PLEASE REPLY BEFORE I MIX . THANKS AND REAMEN BLESSED

Can i use lc instead of spores? Just putting 10cc Liquid culture in the jar?

Could you do this over and over with the same parent syringe, so you have liquid culture forever?

Thanks for the videos im on 15days n there is still alot of spores in my jars did i do something wrong infollowed everything to the t

Thank you

Do you admin 10cc liq cult into 5 lb all-in-one. The same as you would 10cc spore?

So? Mycelium can be broken up and reform, kinda like a sponge? (Sea Sponge can be divided into indiv. cells, and reform) I thought if I broke it up too much I'd be killing some, or at least slowing it down.

Can using too much liquid culture in grow bags prevent mycelium from growing 😢

Just found you, I subbed!

Can you do this without a pressure cooker? Meaning using a different way to accomplish the same thing, not just skipping what it does.

I just washed some millet and tried to use the water with no sugar source we’ll see how much contam I get lol

Your hair really does look excellent today 😂

Thanks for this awesome video, I gotta question, can I use Honey instead of kernal syrup ? If not, what else can I use in case I dont find kernel syrup in my country please

Hi, what kind of nutrients do you use and where to get them?

Does the alcohol not harm the Micelium or the Spores?

Can u just keep the LC in the jar instead of all them 30cc syringes ty 😊

bro look lit af in the begining.

It's always a good hair day for u

I'm assuming I should be using distilled water for the solution? Or is tap water just fine?

Would honey be a viable source of "nutrients"..?

Nice hair. So do you see contamination in the LC or do you just roll the dice? Is it safe to assume that the same thing can be done from LC to LC? How manc CC of LC would you add to 800ml of solution?