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This video is also available in HINDI. Isolation of lecithin | Isolation of Phospholipid from egg yolk (हिंदी में) 1st Video on RU-vid • Isolation of Lecithin ...
Isolation of Phospholipid from Egg yolk
Principle: Many phospholipids are insoluble in acetone and they precipitate out whereas triglycerides, sterol, pigments etc are soluble in acetone.
Egg and egg yolk is very important sources of Lecithin which is a generic term to designate any group of yellow-brownish fatty substances occurring in animal and plant tissues, which are amphiphilic - they attract both water and fatty substances (and so are both hydrophilic and lipophilic), and are used for smoothing food textures, dissolving powders (emulsifying), homogenizing liquid mixtures, and repelling sticking materialsLecithin can easily be extracted chemically using solvents such as hexane, ethanol, acetone, petroleum ether, benzene, etc., or extraction can be done mechanically. It is usually available from sources such as soybeans, eggs, milk, marine sources, rapeseed, cottonseed, and sunflower. It has low solubility in water, but is an excellent emulsifier.
Procedure
1. Collect egg yolk from 2 egg in a beaker and mix well.
2. Add 50 ml of cold acetone and mix with glass rod and allow to stand for 15 min.
3. Collect the precipitate by centrifugation for 5 min at 4000 rpm.
4. Wash the precipitate with sufficient quantity of acetone till the supernatant is clear and colorless.
5. Extract the precipitate with about 100 ml of chloroform-methanol mixture (2:1 v/v) for 3-4 hrs at room temperature.
6. Filter and and collect the filtrate (extract).
7. Evaporate the extract to dryness under a stream of nitrogen or at room temperature.
8. Dissolve the residue in small volume of petroleum ether (10-15 ml) and re-precipitate the phopholipid with addition of cold acetone (about 50 ml) and then allow the precipitate to settled down at the bottom after this collect the precipitate of phopholipid
9. Dry under vaccum and store in dark in a minimum volume of chloroform-methanol system or as solid.
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8 окт 2019
