Omg what a Bullshit blending and such idiocy. Mix water with Sugars (anything you like) honey, agave, brown sugar whatever. Insert Stirring bar, sterilize 15-20 Minutes let cool down . Add any amount of mycelium from petri dish or as i like liquid ones (through an injection port which i put in the lid beforehand along a syringe filter to give it sterile air access even its not really necessary). Then just let it sit, put on the stirring Plate every 2-3 days for 10 Minutes to break the mycelium up and yote ready to go. No need for blender bullshit or similar. Usimg a injection port in the lid allows you to not have to open the jar after Sterilisation so you don't even need sterile air! Drill a hole, buy Gummi injection ports, press in, sterilize. Inject mycelium from another culture or bought one (wiping needle and Injectuon port with alcohol beforehand), stir every 2-3 days to avoid clumping and you're done without any need of sterile air!
What a refreshing character this Walla guy is! Humble, informative, not boring at all and so cool..!! Only 2 minutes into the video, I immediately went to the website and made an order and gather a new hope. I just hope his friends at inoculate the world are everything he says they are. Keep up the amazing work you're doing Mr. Walla! You sure inspired me again. Peace
Thank you so much for making this video! I am relieved and inspired to see someone sharing this information and love the idea for your free mycology school. You are a great teacher and seem to understand the subtleties of expressing information without coming off patronizing or elitist. I hope you so much success, keep inventing!-britta
Oh yeah man. Mycologists and other "craftspeople" have subcultures that are all about the sharing information. It is is like you said about a high tide carrying all ships. Part of that too is knowing there is more than one way to spawn your grain. Your LC is a bit too cloudy for my liking but I see the merits of the extra nutrients, that all is a matter of preference. There are pros and cons to most procedures in the field. I'm lucky I have a friend I can ask questions to and trade back and forth with. I don't believe in karma but when learn or do a subject/skill like this (or microscopy, or photography) I ask dumb questions on the way up to build my skill levels. When I've learned enough to start offering help to people newer than me I like that feeling because it a cool thing to do. I also learned a long time that no matter how skilled you are in a subject there are still things to learn and sometimes a complete newbe will come at a problem from a different angle and have a great idea about how to do something better.
I’ve been researching mushroom lab work for about a year now, slowly buying gear and putting a work area together. I’ve found several amazing channels and resources this past year, and somehow never came across one of your videos! Wow, absolutely incredible work! Can’t wait to go browse the rest of your videos, thanks so much for what you do.
You really don't need all these additives. Mycelium will grow just fine in a solution and honesty, using too many nutrients can cause the mycelium to grow too fast, and you will have difficulty sucking it up into a syringe. I use a simple 2 grams of light malt extract to 500ml of water and its never failed me.
I think what you're doing is awesome, I value this info and find it extremely useful, and I really appreciate you for being above the gatekeeping mentality and carrying such a positive vibe. Thank you so so very mush!!!! Big love for you and the work you're doing in the world! ❤🌍
I leave in Nigeria and I want to be useful in growing quality mushroom adaptable to Nigerian weather being Rainey and dry seasons in a year, how can you help?
i cant yet afford a flow hood , ive tried agar in SAB but every plate got contaminated and i dint know why .. can you explain how to use a bunsen burner to create a decent working area ?
I was so sceptical when you threw the whole Agar plate into the jar thinking that this plate could innoculate 10s of other jars but looking at the innoculation results and the time it took I am mind blown And i dont even grow mushrooms yet, been into heavy research past month and this single video of thinking outside the box is really mind opening. Does Paul Stamets know about this? :P
Just as a reminder, alcohol IS quite flammable. BE VERY CAREFUL WHEN SPRAYING ALCOHOL NEAR A FLAME. Move your hands away from the flame when spraying people, I cannot tell you how many people since the pandemic set themselves on fire using hand sanitizer and then lighting a cigarette.
On your "other technique... Just scratch your knive on the agar surface and skake your knive in the liquid abd yore done even you didn't even see mycelium on your knive! No need to put any big amount in the liquid culture! Scratch and put 1 micrometer mycelium in and it grows!
Incredible I always thought this would be too expensive and take too much equipment for me to do in my small living space. You've changed my mind. Much appreciated.
Wow… this dude used the whole Petri dish in the lc… lol others are like oh just a drop.. yet his grain bags grew super quick so .. clearly he’s on to something. The more you use the more mycelium your grain bags will have faster.
Yep that’s the idea! By using a lot of mycelium, the LC will also grow more quickly and there’s a much narrower window of opportunity for uninvited guests
Thanks for this. Very informative, great practical demo and comprehensively explained. I find your attitude and philosophy inspiring. I've found this a lot with mushroom growers. Great work bro 👍
I found your channel randomly, but I’ve been using InoculateTheWorld for about the last year. I cannot recommend them highly enough! Their isolated syringes are amazing.
Dang, this was helpful! If you can make videos about troubleshooting common issues with growing in bags on masters mix that would be great. Identifying contam, how to tell if the substrate is too wet or dry, etc. Thanks!
If I may pick your brain...? Is peptone useful? Good to use? Doesn't putting the LC in the refrigerator slow growth? And your info is precise and needed in this field of disinformation. Some info is flatly wrong or remedial. Your delivery and amount of useful info is greatly appreciated.
I don’t know, I’ve never bothered with peptone. I know some folks swear by it though! LC is definitely fastest when fresh, but it stays active for quite a while in the fridge. It will gradually slow over time, but as long as it’s clean it can last many months. Thanks for watching!
This is rad. All of this. You're rad, your presentation is rad AF, easy to follow, easy to hear (aspergers is a bitch sensory wise sometimes). Anytime I come across user friendly, community sharing based mycology "how to's" I have to acknowledgeit. You aren't hoarding the basic knowledge of mushroom growing like the boomer generation of mycologists before you who think sharing growing tips and tricks is highly discouraged and that women don't belong anywhere near a lab, or included in shop talk. Soooooo refreshing to find a spot where that isn't the case. Dope AF bud.
Amazing video. I believe you will be great professor and i want to enrol in your course for mushroom cultivation. I have recently been reading how fungi can be very beneficial to health.
Great video, thank you! However the comment about "generally a good idea to add water to the pressure cooker" is frightening. One MUST have water in the cooker.
A-MA-ZING! Thank you so much for your shares. ......maybe you know also something about using EM or other congregation of microorganisms to enhance productivity or to have a more safe substrate for mushroom mycelium? Because a grain micelium seller told me to mix the grains (outdoor) with non sterile substrate, and EM to make the things going.. and I would be very interested to know how much sense it makes!
I just have to tell you I love your channel your content and the way that you move around and do things it inspires me I'm old now but I still love to take this type of medicine makes me feel whole I see the world clear and I see people clear anyhow I love it and I love your channel thanks for putting it out
the mason jar blender is interesting. i have never seen anyone use that before. from my understanding though, wouldn't a stir plate and stir bar be able to break up the agar? just not as quickly?
Does liquid culture have to be continuously stirring/moving? I have a magnetic stirrer, but my question is does it have to be on and stirring constantly?
Great question! No, in fact you can do away with the stir plate altogether: many folks use a marble to aid in mixing and simply swirl by hand once or twice per day. However, constant stirring has some advantages: it prevents clumping (large chunks of mycelium can form on the surface, which can be challenging to pour cleanly and reduces inoculation efficiency), it accelerates growth A LOT, and significantly increases oxygen, which aids rapid growth and reduces chances of contamination.
In the case of the fungus an sterilized media is not necessary. In fact in a rainforest nothing is sterilized. Some grows up only in cow mole. Others only in horse mole. Other where the donkey urine. Some over rotten wood. And other under the leaves of certain tree like pine. So the selectivity is determined for a bacterial culture and the nutritional needs. Studying the soround of the misellium, and the fruit foot soil or substrate and reproducing this bacterial culture, pH an nutrients is possible sprouts of spore without keeping sterilized the media during the process. The only need would be a cloth or covet to avoid fall dust in it. In fact if it is not real any mushroom cultivation would not have success. So prepare a media to transfer with proper bacterial culture make easier the success of grow up with less care. The base in common cases I think would be acetobacter, lactobacillus, some bacillus, azotobacter. With this spores of agaricales a basidiomycetes sprouts like in a grass healthy soil. If not achieve a proper culture the problem becomes smaller things like in laboratory and hospital: mycoplasma, virus, viroid. www.marknature.com/products/bacillus-amyloliquefaciens?_pos=2&_sid=c0d14ac15&_ss=r www.marknature.com/products/azotobacter-chroococcum?pr_prod_strat=copurchase&pr_rec_id=ce388297f&pr_rec_pid=6803070288047&pr_ref_pid=7254536781999&pr_seq=uniform
So, do you think it’s viable to pull some mycelium from an already spawned grain bag to make a culture with to start new spawns? or should I inoculate from spores into agar and then make solution.
You keep saying how to keep from cross contamination from yeast but you put some in your solution before going into the autoclave or pressure cooker, what do they do?
Thank you so much for sharing the knowledge, been having a hard time progressing my knowledge of all the processes of growing mushrooms because it seems many do not like to share. Do you have any resources I could read up on when making a LC ? Or are your mixes something you created on your own? Again thank you for what you do, great video
Thank you! I developed this process through tons of trial and error. A great place to start is Radical Mycology by Peter McCoy. So glad you liked it, stay tuned for more!
Thank you for your philosophy. I have a dream to start a mushroom farm in the inner city and teach anyone who is interested. Feed the community - support the community.
🙏hello I am new on all this I will like to know if you are giving classes on how to grow I will love to attend one of your classes and where are you locate I am in Phoenix Arizona thank you for sharing 👏❤️🙏
Hi Jackie! Yes, I’m teaching a cultivation course in Walla Walla in September. It’s an 8 day retreat in a beautiful place with great food and lodging. You can sign up through Fungi Academy (www.fungiacademy.com)
Love peace kindness is power you're not getting a trade secret away you're just teaching people high tides Ray ships just like everyone else we all find our way do we all have something to do I just want to say I love you brothe❤ thank you you're teaching people the knowledge they need to know
I am a beginner in mushroom cultivation and this video may helps me a lot. Thank you so much for sharing a free learning techniques. Happy mushrooming🍄
Wow. You put a LOT of ingredients in your liquid cultures. How often do you have to deal with contamination? I assume you always have to test it on agar after making an LC since it would be hard to check by clarity. I mean it's always good to test on agar anyway but.
As long as the transfer is smooth, the culture is clean and the filter does its job, contamination is very rare (My average is probably about 4%, usually from sloppy transfers). Using microscopy and/or a streak test are the only ways to know if it’s clean, imho. I’ve seen plenty of batches that looked great but were totally spoiled, and plenty that looked off but came out perfectly clean.
sounded great, so i bought the blender, but the blade of the Oster blender are 2.59 in diameter and the hole of even the smaller of the mason jars is 2.75 " diameter. how is it not going to fall in?
Hi, thankyou so much for this awesome, insightful video! I am so interested, and also wanted to ask. Would this be ok to work in a bus while i travel? Would the fungus might infest through my bus? Thats what im worried about. And how long would the liquid culture last?
Yes, you can definitely grow mushrooms in a bus! Mold is a problem, but gourmet, medicinal and therapeutic mushrooms have very little risk of spreading where you don’t want them to. Liquid culture will work as long as you are parked for a few days while it brews, otherwise it will slosh around and soak into the air filter, which will cause problems. Clean, healthy LC can last for months!
Now this is a pretty useful video! Thank you a lot. I have a small question, I'm a beginner student on mycology and I still haven't completely grasped the concepts of spores vs LC. Would you say that the process can be summed up with 1) get the spores from the fruit body into a syringe 2) inoculate a petri dish with the spore syringe 3) once you get mycelium, inoculate LC with it and then 4) produce grain spawn once you confirm your LC isn't contaminated?
Yeah that’s a good process, although I would skip the syringe. You can just scrape a few spores from your print onto the petri dish. I use a loop and a drop of sterile water to smear the spores around. The resulting growth represents numerous spore combinations, so it usually takes about 3 transfers til you have an isolated culture. I’ll be doing a video on this soon!
