If you know you're going to store long-term, and want to purposefully stall out the mycelium, you can go without the air exchange hole. It's great for growing the mycelia quickly, but sometimes it grows too much. They won't die, they'll just grow very slowly, which is useful sometimes. 🍄
Tip for the application of the silicone. Use a wet cloth, or paper towel and press the silicone done. It will leave a nice and fine blob and will not leave a widespread mark of the silicone.
I spit on my fingers n wipe it. Who cares what way as long as works for u. But a great point nobody else makes is that by pressing it down/wiping it flat it makes guiding the needle thru the hole a doddle. Because it will be easier to see exactly where to guide it properly without hitting the metal lid(edges of the holes made)
IMO honey gets too cloudy and here’s my simple LC recipe. 1g light malt extract 1g peptone 600ml water Dissolve and sterilize. Works great & very clear only mycelium shows.
I really want to see an example of how fully colonized LC looks in a jar. Plenty of videos on how to make LC, but no one ever seems to tell you how to tell when its ready to draw up into a syringe.
Thank you! So nice to see a vid using honey instead of corn syrup. How long does the LC stay good for once it's made and does storing it in the fridge extend shelf life?
@@HappyMushrooms dang, 3 years! I ask bc Ive heard the myc starts getting weaker after a few months unless you start a new batch and feed it more food. Maybe that's partly genetics too. Guess I'll have to try it out and see. Thanks again! 🖤
@@whitneylee33 Yeah, you need to give it a weekly stir. Basically, don't let it go stagnant. Not that it would completely, having continual growth occuring.
thank you my mushroom friend.i have a question for you.i ate all the cucumbers now i want to make liquid culture from honey water.i have 10 cc syringe with spores dissolved in water.can i use your technique to inoculate?how would you go about it?thank you very much.
Thank You for a good video. One question regarding storing temperature of the LC after injecting the mycelium. For a longer time it should be kept at 6-8 degrees C but what about the initial growth phase. Warmer for getting the mycelium to take of?
When I add honey to distilled water it instantly becomes cloudy even before pressure cooking it. Maybe it depends on the honey I use? Mine is organic honey.
Hm... I also used organic clear honey from a local beekeeper, but "normal" water. Maybe its the honey, but I have only tested this type of honey so its difficult to tell
You really don't have to be concerned with the cloudiness. If it's sterilized then your good. Some or most of that will probably go away after several days and your growing a culture, not a clear glass of liquid so I personally wouldn't worry about it. You can also filter it through coffee filters before cooking and heat rid of some of that if you want.
I am gonna try this, but in the oven, saw someone do it when making spawn, i woder if you know anything about it? I feel like im gonna blow something up if i do it wrong. i don't have a preasure cooker.
I bought some growing kits, spore prints, spawns, liquid culture and psychedelic products from an online supplier and they are affordable and discreetly delivered
@@HappyMushrooms That's awesome 👍. I started watching your videos because of the mushroom time lapse and then because of your photography tips and I just discovered your mushroom videos last week. Thank you for putting out great content!
Do you incubate it at a certain temperature? And how long can you store it? Thanks for the videos, i just stumbled upon your channel and find it very informative!
Hey, yes, I incubate at 21°C (70F) in a tent with temperature control. Great you like my videos:) Storing... dificult to answer: I have petri my mycelium in my fridge which work after 6-9 month. I also had grain spawn which started to grow mold after 3 month... Best way is to put it into fridge after 4 week, but I do not have so much space so I lose grain spawn from time to time if I wait too long (or it just starts to fruit)
Im currently enduring super hot temps where I live and my shop, where I keep the stuff has been getting above 80F -the liquid cultures I have really thrive well at these temps but I also know you can put them into a fridge to stall out the growth just make sure you stir or agitate somehow every couple days so you will be able to pull out syrige-fulls easier as needed.
@@codelicious6590 I'm not a professional I just love them. If memory serves me. Some mycelium, mushrooms or microhorrzial species are more heat resistant. Where I'm at climate zone 6 rusula and oyster mushrooms thrive here. Also Ive seen amanita veroses and vaginata. In 90°f weather here
Hay why not just microwave the jar until a rolling boil put on the lid on with no holes and flip upside down. Heat from the liquid will maintain 180f+ for more then 10min and this should be sufficient in killing spores and bacteria. Just a thought
It’s enough to pasteurize but not to sterilize. Sterilization requires 130 degrees. Water can’t get hotter than 100 C at sea level because it will turn into gas. You need the pressure to raise the boiling point of water so that it’s still liquid at 130 C.
Danke für das tolle Video, Jens :) kannst du mir sagen, warum man nicht einfach das Watte Loch als self-healing injection port nutzten kann? Die Watte geht doch genauso zusammen, wie das Silikon, oder sehe ich das falsch? LG aus Dresden, Thomas
Danke Thomas, ehrlich gesagt... habe ich mich an die Anleitung anderer gehalten. Ich muss hier raten: Die Watte könnte verunreinigt werden, ein Loch könnte entstehen (das könnte man natürlich wieder schließen). Wenn die Watte dicht genug "gestopft" ist sollte es damit aber eigentlich auch klappen, zumindest fällt mir spontan nichts ein was sonst noch dagegen sprechen würde. Bei Mehrfachverwendung ersetze ich die Watte, den Gummi/Silikon port nicht. LG aus Braunschweig
I saw a lot of other growers just using boiling water.. but they boil for 10-20 hours instead of 45-90minuten. I think it will work, but I have not tested
@@HappyMushrooms There is also Tyndallization method. I've read different versions, but one I've used is to boil for 30 minutes, then let cool, and the next day reboil again 30min, for three days in a row. Some people think this is much too slow, but I think compared to the incubation period it's not bad at all. For a beginner, taking a little bit of time after work, for example, it's a good way to start without a pressure cooker.
Hold a pan lid down by finding a way to weigh it down safely is what i did on my first year, but still allowing for gas exchanges. And i boiled in pan for 3hrs. Ive done grain/LC/agar/manure this way. Never failed once i hit the 3hrs mark. But u gota not overfill pan and top up every 20-40ish mins. It’s never been successful on 100% of sterilisation when less time is applied. Make sure all glassware is already sterile before u make the LC up coz i encounter contamination with old used food jars when i don’t pre sterilise them prior to LC attempts
@@antoniosa932very true. As I actually use alot less that u. I simply just get a cluster of LC mycelium into syringe which on occasions its been as little as 0.2ml when using a 1ml syringe/18G needle end.
That very last jar shown on the last few seconds in the video,,,,,,, is that contaminated?? It looks different to the others colours and consistency. I have a jar that looks similar. Thats y i ask😢