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How To Normalize A Heat Map In GraphPad Prism

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In this video tutorial, I will show you how to normalize a heat map.
Without normalization, plotting raw data onto a heat map can make it difficult to interpret the result. This is often seen in the case of gene expression data. Normalizing the data before plotting the heat map can make it easier to interpret, compared with using the raw data.
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Опубликовано:

21 авг 2024

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Комментарии : 19

@brianlweiss 8 месяцев назад

That was a ery useful video, thank you!

@mrsaleemohd Год назад

So helpful. Thank you so much for nice explanation.

@AqleemAbbas 4 года назад

Sir, Great. We and my other collegue request you to shed light on publication figures using powerpoint or photoshop. For example how to adjust 30 petriplates on a big slide of powerpoint (56 * 56) and then to export to word in a way that fonts & quality of the figures would be exactly what the Journal wants. If It is possible kindly make a video.

@guanhuang4506 3 года назад

Thans, very helpful

@bindup9219 4 года назад

Hi can you please make videos on AUC curve and ROC curves. I appreciate your help. Thank you

@StevenBradburn 4 года назад

I will be making a start on these in a few days :)

@AqleemAbbas 4 года назад

@@StevenBradburn Sir, Your Videos are Great. My collegues & 1 request you to shed light on publication figures using powerpoint or photoshop. For example how to adjust 30 petriplates on a big slide of powerpoint (56 * 56) and then to export to word in a way that fonts & quality of the figures would be exactly what the Journal wants. If It is possible kindly make a video.

@zeeshanqasim8925 4 года назад

good video sir. 1. I am wondering, how we can specify the significant differences among the data sets 2. Is it possible that significant differences can be displayed on the heat map as well? 3. I have seen on various publications, they demonstrate the dendrograms alongside the heat maps. How we can add dendrograms here sir. Thank you.

@StevenBradburn 4 года назад

Hello, 1. You could add an asterisk to demonstrate any significant difference? 2. Same as 1 3. So, it is not currently possible to create dendrograms with Prism. You are probably best looking at R to do this I hope that helps Steven

@zeeshanqasim8925 4 года назад

@@StevenBradburn, thank you, sir.

@zeeshanqasim8925 4 года назад

@@StevenBradburn Sir, can you please specify, how we can add an asterisk to different colors?

@StevenBradburn 4 года назад

You could use the text tool and change the font color to suit your needs?

@arne9099 3 года назад

Thanks for the nice video. I got gene expression data from RT-qPCR and already did the normalization with my reference genes to obtain fold changes. Is it then necessary to perform the normalization in prism?

@rizwan3147 4 года назад

Sir very nice I want to ask how I can draw an error bar in multivariable data? for example, I have 6 treatments for wheat crop yield and its parameters. please teach us about agricultural-related graphs like fertilizer and irrigation treatments effect on yield and yield parameters, thank you Sir

@rhasanvand1359 4 года назад

Hi Thanks for great video. How can do heatmap for large data set like gene expression data? graphpad prosm dosent accept large columns it has limitation

@kinetomagnetic Год назад

Hi, Can we sort the data in this software to represent only top 20 or top 10 species? Generally in NGS data it is not possible to plot all the species (because of large number of species).