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How to Prepare a Single-Cell Suspension from Primary Tissue Samples (e.g. Mouse Spleen) 

STEMCELL Technologies
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This video demonstrates how to harvest cells from a mouse spleen and prepare a single cell suspension prior to performing cell isolation. Preparing a single cell suspension from a primary tissue sample will enable cell separation by minimizing additional cell loss and improving the labelling of target cells.
View a written protocol on how to prepare a single-cell suspension from mouse spleen: bit.ly/32h1zAw
Find information on how to prepare single-cell suspension from frozen samples: bit.ly/2HZ42Je
Learn more about cell isolation from intact tissues: bit.ly/2I4Jkr1
#CellSeparation #CellIsolation
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29 авг 2024

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Комментарии : 10   
@ellocoassis
@ellocoassis 9 лет назад
Good, really hepful. Waiting more videos about cell culture!
@junzhao1079
@junzhao1079 7 лет назад
very cool! have you try this method in primary neuron culture?
@Mrd0c26
@Mrd0c26 11 лет назад
great video, very helpful
@oscarguadarramaveterinario8415
Do you have any information about fat derived stem collection techniques?
@kavitarana9330
@kavitarana9330 5 лет назад
Can any body suggest me the reciepi of mincing solution for hard tissue like kidney
@pathologist1978
@pathologist1978 7 лет назад
Is this preparation suitable for flow cytometry ???
@pathologist1978
@pathologist1978 7 лет назад
Thanks
@karthikv.k8203
@karthikv.k8203 4 года назад
What is dissociation medium??
@STEMCELLTechnologies
@STEMCELLTechnologies 4 года назад
A dissociation medium contains enzymes, and is designed to maximize the viability and recovery of dendritic cells from mouse spleen when used with cell separation technology. If you have more questions, please feel free to email techsupport@stemcell.com!
@karthikv.k8203
@karthikv.k8203 4 года назад
@@STEMCELLTechnologies Thank you
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