How to select the column dimension of an HPLC column? 

Sir please make a short video on method of preparation of buffer of different pH with calculation

Regarding poresize, you mentioned 1000A to 4000A for higher molecular weight of protein and vaccine, Need to understand what is the molecular weight of protein and vaccine? Also, please mention this is for purification related,ie downstream process. For Analytical estimation purpose for higher molecular weight, example upto 150 KD we can use 300A for LC.

Nice sir, Sir.....is wider pore size columns having Higher surface area or lower??

Good morning sir Which column is suitable for surfactant molecule. I am getting four peak on chromatography.

Sir, i am from bangladesh. I like your video. I want a video related to elemental impurities.