How to sequence the human genome - Mark J. Kiel 

Oh come on mann u have a lab and learning from RU-vid.. 😂😂😂LOL

I didn’t understand much… could please summarise here

@@PrinceKumar-zs8dh Max x100 full sequencing runs per week...

@@wadudahmed9107 maybe they're not the scientist. Just the facilitator of lab and equipments

@@wadudahmed9107 dude you are here too

$100 bucks next year: www.cnbc.com/2019/07/01/for-600-veritas-genetics-sequences-6point4-billion-letters-of-your-dna.html

Hmm, if the car had of followed the same trend, then a Ferrari would cost less then 40pence(50 cents approx in the us)... and so... I’ve found a penny on the floor... I’m off to get a new Austin Martin, a penny a month with a 100z interest rate for each month, fine by me.

I wouldn’t give a gypsy lady a thousand bucks to tell me my future, why would I give someone a thousand bucks to tell me my past

It’s funny to think that humans were able to evolve so fast but devolved from karens

@@AngerPacifist nope

BNGO is going to revolutionize this process folks $$$🤑

Thank you for this. I had no idea.

We come a long way since then and achieved 100% genome sequencing in 2022

I will study bioformatics next year 🙂🙂

Fatiha B. Ayyyy aghase 💚

i guess you went to a lousy college then XD

Hence, That's where we are heading now...

Yeah nice observation, that movie is great btw, but watching it made both motivated and insecure

CTAATGT

@@omkarchavan5940 hope Neo will reload the Matrix :)

@@omkarchavan5940 GATTACA portrayed a technology that can predict specific personalities and everything phenotypical (body characteristics that are observable) which was a predominant theory in the 20th century, known as biological determinism. However, as science advanced, the theory had became outmoded and invalid, primarily due to the current acceptable scientific view of the world which is stochastic. Moreover, we had found that protein-coding genes are at times, multifactorial and require the environment and social interactions to shape its function, which directly contradicted the ideologies put forth by GATTACA.

Yes

^ this is such an AAAAGTCCCCTA thing to say ^ And here in lies the problem with sequencing.

hmm.........It's like a program.. ..like 0's and 1's.

Saloni Bhurke Exactly.

Saloni Bhurke *In Arnold Schwarzenegger voice* "Who is your programmer?... And what does he do?"

it was the best of times, it was the blurst of times.

This comment aged so well, PCR is extremely important right now.

@@AnimMouse literally how we were able to make a covid vaccine as quickly as we did :)

@@okliam i like how you replied

@@okliam 8 years man..

MAYBE SHE FORGOT HER ANATOMY

maybe

It really is a very tough subject, i nearly flunk on it.

I like the Subject

Good for u girl. hope that someday you 'll become a doctor.

Well, there are chemical reactions that produces colored compounds. That's what those enzymes do, they catalyze a reaction (e.g. break something down) to produce some colorful chemicals. That's the color part. And no, A and A or any of the other combinations usually don’t bond together because of their unique chemical properties. But yes, sometimes mistakes do happen. For that, a single sequence (the small parts mentioned here) is read more than a hundred times to ensure that those "by chance" interactions don't affect the final result.

no, we do not function from binary structure- and we are made up of different parts. Our brains do not function the same way, nor do our cells. If you consider a computer program as anything with information, then yes- but thats like thinking anything with wings is a bird. Humans do not work like computer programs, and even neural nets cannot function with the same braod complexity that we do.

Antony Arango DO NOT OVERTHINK IT.......YES WE ARE TECHNICALLY COMPUTER PROGRAMS (more like a collection of it), IT IS THE SAME IN THE SENSE THAT A STRING OF LETTERS WILL BE (in essence) CONVERTED INTO SOMETHING USEFUL. ANY MANIPULATON OF THE GENES WILL CHANGE THE PROTEINS THAT MAKE UP YOUR CELLS (just like any manipulation of the code will change a program). A DNA CAN ALSO BE CORRUPTED MUCH LIKE IN A COMPUTER.

Price Where are the behavior markings in DNA?

its not coding language its adenine guanine thymine cytosine

@@Cpt_John_Price If we were computer programs, as put forth by you, then it is reasonable to assume that increasing organismic complexity positively correlates with complexity of the genome (total DNA information), right? Now tell me why single celled microscopic Amoeba have 223x more DNA than we do?

gr8 m8 no h8

Bioinformatics probably

Well, the binding of one nucleotide (the A,T,C and G) can only occur to a single strand after the previous one is added. Also, the pairs are constant. so where there is a T, nothing other than an A can bind. Very low chance of mistake. But of course, mistakes can and do happen. That's why a single sequence (the small parts mentioned in the video) is read more than a hundred times. So that those accidental mistakes don't affect the final result.

Depending on what you are 'stitching' the genome together for. Just say you are sequencing another human genome to confirm the dissimilarities between them, they can always use a reference genome, aka an already sequenced human DNA, to help piece the DNA together. Now, no human DNA is the same but there will be massive similarities so you can still use that to help solve the problem you mentioned so as they do not fall in the wrong place. Also on another note, all those smaller pieces are different so it will be an overlap over an overlap over another overlap so it is not just an overlap of ending sequences. Another way to look at it is that these are more than billions of small fragments, or a shattered DNA, and not just 1 DNA but a pool of DNA of the same sequence. However, it is still not that perfect and needs to be sequenced enough times to get a solid answer before getting published. Third generation sequencers are trying to solve that problem, though. So instead of shattering DNA into smaller pieces, they can sequence the whole genome without breaking them up. It is still really pricey though.

Thank you for the answer.