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How to use AlphaFold2 for Wet Lab Researchers 

Reza Rezaei
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28 окт 2024

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Комментарии : 17   
@orhunakengin7072
@orhunakengin7072 Год назад
Such an underrated video, thanks for explanation!
@maxhuber1361
@maxhuber1361 Год назад
thank you so much!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! I am waiting for the continuation
@lunanaw5867
@lunanaw5867 3 года назад
Thanks! Great explanation.
@Maria-gb8bv
@Maria-gb8bv Год назад
Thank you!!
@shrutiagrawal8026
@shrutiagrawal8026 11 месяцев назад
at the time of installing the database is shows error "Error: aria2c could not be found. Please install aria2c (sudo apt install aria2)." in spite of having aria2 installed.
@jamesbean9396
@jamesbean9396 3 года назад
The champion of computer illiterate wet lab researchers xD Cheers mate, great video!
@RavindraThakkar369
@RavindraThakkar369 2 года назад
I have two peptides with 17 residues each, one is head to tail cyclic peptide and the other is stapled peptide. Does alphafold2 work with chemically modified peptides?
@dandeelyonn
@dandeelyonn 3 года назад
Hey Reza, could you please make a tutorial for using rosettafold please? Maybe that's possible to run locally since the requirements are lower?
@rezarezaei4868
@rezarezaei4868 3 года назад
I think there is also a full online version of rosettafold that I can record a video on it and for that I don't think there is any need for a local version. For alphafold the local version uses the full database and the online one uses a smaller database. So, the local's result is more accurate in some cases.
@dandeelyonn
@dandeelyonn 3 года назад
@@rezarezaei4868 hi Reza, Yes I did some digging yesterday and found that the Baker lab website has an online submission point so it is easy enough to look at individual proteins- however I am unsure of how to build complexes (I heard that if you just add a flexible linker and repeat the monomer N Times that can work, so might try that with the server submission)
@deby6293
@deby6293 3 года назад
Hello Reza what to do in case you want to predict a dimer?
@rezarezaei4868
@rezarezaei4868 3 года назад
Hi, actually I haven't tested this myself but I read that if you put a monomer first and then a hundred X and then the sequence of the second polypeptide, you can predict the dimer structure. But I'm not sure how accurate that would be. There have been some interesting results using this method! However, the current version does not natively support dimers.
@premlatamajhi7059
@premlatamajhi7059 Год назад
U should have made it clear for those who don't have any dependencies what should they do
@omidmokhtari4606
@omidmokhtari4606 Год назад
"damet garm" from Stockholm xD
@marcelosilvafolhasdamas1329
@marcelosilvafolhasdamas1329 3 года назад
What are your computer parameters? The minimum requirements for running it?
@rezarezaei4868
@rezarezaei4868 3 года назад
I think the minimum is at least 8 gb of gpu and 32gb of ram. Also you need around 3Tb of fast storage for having a local copy of all the libraries.
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