Immunohistochemistry | How to perform immunohistochemistry? | application of immunohistochemistry 

this was so much more useful than anything I've learnt at uni! really great visuals and great explanation! THANK YOU

Immunohistochemistry (IHC) techniques involve the use of antibodies to detect specific proteins or antigens within tissues. The process involves the binding of primary antibodies to the target antigen followed by a secondary antibody conjugated with a detectable marker such as a fluorescent dye or enzyme. This allows for the visualization and localization of the target protein within the tissue sample. An immunohistochemistry test involves the application of IHC techniques to detect specific proteins or antigens in tissue samples. This can be used to identify specific cell types, diagnose diseases, and monitor therapeutic responses. To perform immunohistochemistry, tissue samples are first fixed and sectioned onto slides. These slides are then treated to remove endogenous peroxidases and block non-specific binding sites. Primary antibodies specific to the target antigen are then applied to the slides, followed by secondary antibodies conjugated with a detectable marker. The slides are then washed and counterstained before being viewed under a microscope. The components identified by immunohistochemistry can vary depending on the specific target antigen being studied. This can include proteins, hormones, enzymes, and other biomolecules. Immunohistochemistry is used for a variety of applications, including the identification of specific cell types, the diagnosis of diseases, the monitoring of therapeutic responses, and the evaluation of research hypotheses. IHC stands for immunohistochemistry.

I'm training to do this at work, thank you for breaking it down to the basics!

Your brief and lucid explanations are THE BEST!!!

Very Nice thank you! Would have been great to have more details about Blocking! And the different animals used: mouse or goat or rabbit! But very useful and clear.. Thank you!

this is what I needed, just quick, concise, but good explanation. thank you very much :)

Excellent synopsis of the concept and process! Thank you very much!

Very helpful! I shared among my friends.

Today I saw your first video on immunoblotting and after that i covered all my basics from your rest of the videos.. Literally, mind blowing video, content and voice too. Thank you so much... Looking forward for your more videos on microscopy and spectroscopy techniques.

Thank you so much, very clear and easy understanding !

Thank you. This was a good summary.

This is a super overview! Thanks so much.

very well explained thank you very much!!

You explain everything perfectly ❤️

Better than professors' explanations, very clear overview! Thank you

Marvellous 💫

thank you very much

Thanks, Comrade.

Explained the topic very well !❤️

I have a question regarding colorimetric detection: for the secondary antibody to work, in the cell there must be a process that is regulated by the enzyme attached to the secondary antibody, right? And does this process have to do with the original protein that interests us, the one that is recognized by the primary antibody? Thank you

U helped me a lot!!! Thank you. Neurology resident from South India

Presented very well and cleared the concept

Simply put and neat.

Awesome, Thank you!

THANK YOU!

Thank you! Good video!

Lovely explanation.tnx

Legend! Thanks for your video :)

Thank you very much, sir.

Nice.. Keep it up

U are really good teacher.. __. Ur follower from Jordan

What are the methods used for signal amplification in IHC?

Thank you so much

Great

What is diffrence between IHC and elisa and the best techenique to be used to determine enzyme presence

Bhai biotechnology or biological science k liye same study plan Kam karega ya nahi

Nice

Can u say me the correct strategy preparation (which topics to cover)to clear IIT JAM with a month ?

im in highschool ( gr 9 ) trying to act like I understand everything explained here. lol

what is the difference between Immunohistochemistry and immunofluorescence

Why are doing secondary ab incubation in dark?

well explained I think you explained Immunocytochemistry instead of immunohistochemistry

This explanation is much more immunofluorescent than immunohistochemistry.

is fluorescent an enzyme?

nice video

Please do not confuse between immunofluorescence and immunohistochemistry? What happened to antigen retrieval? this video can misguide people

i like your video, but I think this is not a Immunohistochemistry, this is a mmunofluorescence tecnique.

Huge mistake..please before u upload the video cross check..Epitope is a part of the antigen..not antibody..part of antibody name is paratope..

Sir, Thank you for this...