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It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️
well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain