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Indirect ELISA: Animated explanation 

Sajid Shaikh
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Reference: Immunology by Kuby

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7 авг 2024

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Комментарии : 128   
@animal_expert
@animal_expert 2 года назад
Thank you Sir! Very helpful that you included the case where the primary antibodies aren't specific to the antigens to see the comparison.
@kyramirchandani7270
@kyramirchandani7270 4 года назад
You explained the toughest part in simplest way.hats off sir
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Thanx kyra. If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm/Google pay number 8830061003.
@nicknicky2001
@nicknicky2001 4 года назад
THANK YOU SO MUCH! Your video was fast, simple, and informational. I appreciate you.
@anna-kb9mn
@anna-kb9mn 4 года назад
You’re amazing! Thank you so much :)
@moniraafrozmoli2626
@moniraafrozmoli2626 5 лет назад
best ever explained...thank you for explaining it so well.
@nathanrussell1538
@nathanrussell1538 3 года назад
A really precise and pedagogical explanation. Well done.
@homosapien682
@homosapien682 4 года назад
The Best Ever Explanation One can Get. Precise
@joan-b-empire576
@joan-b-empire576 Год назад
Thank you from Cameroon 🇨🇲❤️
@shatikkulaminh643
@shatikkulaminh643 5 лет назад
Best immunology video I hv ever seen. .great job ..😊😊😊
@DejavuDejavu
@DejavuDejavu 5 лет назад
It's well explained and it's really helpful. Thanks for the video and hope you can do more. LOVEYOURSELF ♥️
@debdeepchakraborty8859
@debdeepchakraborty8859 4 года назад
Your lectures contains more amount of data , your vocals are easy for us to understand ..... 💓
@sharonjitkaur8411
@sharonjitkaur8411 6 лет назад
what would you say the ease and speed of the process is? how long does it take to perform indirect elisa?
@abelurbina2003
@abelurbina2003 3 года назад
It is clear now that when the chromagen interacts with the antibody with an enzyme the chromagen dissolve and produces colorization in the entire glass. What I am thinking before during the interactions of chromagen to an antibody with an enzyme, the small portion only of the antibody will only produce colored light.
@nyawirawaithaka4993
@nyawirawaithaka4993 4 года назад
Excellent explanation! Thank you
@kvnkvn9191
@kvnkvn9191 5 лет назад
What an explanation!
@jatinjacks1449
@jatinjacks1449 5 лет назад
Well explained! Hats of to you sir.
@Mohitthakurofficial
@Mohitthakurofficial 6 лет назад
Excellent. Your video explanations are very simple and easy to understand. Your method of teaching is quite impressive. Please upload more videos on other techniques as well. I subscribed. Your channel need more subscribers.
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Thanx Mohit ... i am in the process of uploading
@namitma
@namitma 6 лет назад
The sample we are putting in the titer after the coating buffer. Is the sample just the blood of the person?
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Its not whole blood... it is serum most of the time
@user-jd9ex4rp5d
@user-jd9ex4rp5d 4 года назад
Best explanation I swear the doctor who give me the lecture don’t know what to do I red the lecture for for 6 Hours and didn’t understand what my doctor was saying While you explain it in few minutes You are the best♥️♥️♥️
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Most welcome
@sugarboo9544
@sugarboo9544 5 лет назад
Nice presentation, very easy to understand. Thanks a lot.
@abidabibi3317
@abidabibi3317 4 года назад
best explaination and helped me before my presentation 😊
@rubabk1603
@rubabk1603 5 лет назад
Very well-explained! 😊 👍
@ssss...7896
@ssss...7896 5 лет назад
tq so much sr..concept clear n easy explanation
@sciencenerd752
@sciencenerd752 4 года назад
this topic wasn't even clear in wilson and walker but you explained it very well. thank you
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Thanx.. would you like to help our channel by donating small amount (Rs 5 to 100)? This will encourage out team to make more videos
@hemantk345
@hemantk345 5 лет назад
well the way you teaching is awesome great work buddy keep going..... i would like to add a question that after binding of antibodies to antigen ( specific non covalent reaction occurs between Ag-Ab i.e epitope and paratope respectively) the secondary enzyme linked antibodies binds to primary antibody of fc region but as far i know two antibodies cannot react and binds each other then what basic principle holds this situation?? looking forward to your reply thank you
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
As u said.. that antigens have epitopes... so when our antibody is injected in the lab animal, that antibody acts as antigen for that animal... so this antigen (our antibody) will also have epitopes on its surface including fc region also which are called isotypic epitopes... so lab animal can produce antibody aginst these epitopes
@hemantk345
@hemantk345 5 лет назад
@@sajidmicrobiology thank you soo much buddy
@omarhalimi1685
@omarhalimi1685 3 месяца назад
This was so great
@m.gailstotler525
@m.gailstotler525 4 года назад
Excellent explanation/instruction. [Antibodies is misspelled at 6:15 on the video.]
@ciroesposito5395
@ciroesposito5395 2 года назад
Is it possible to do the opposite by putting the antibody bound to the polystyrene plate to determine the antigen concentration in the sample?
@sajidmicrobiology
@sajidmicrobiology 2 года назад
Yes
@ciroesposito5395
@ciroesposito5395 2 года назад
@@sajidmicrobiology ok thanks
@thaniabremer7255
@thaniabremer7255 2 года назад
Very clear discussion!! Thank U Sir:'>
@MdAli-rc3mc
@MdAli-rc3mc 5 лет назад
Assalamoalaikum. Brother! I enjoy your lecture very much. Please keep it up. May Allah livelong you. Thank you
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Walekumasslam... most welcome
@aliraza-hc4nw
@aliraza-hc4nw 5 лет назад
veryy hardworking masahaalh keep it up sir ap electrophoresis pa bhi video upload krya
@sukanyachakraborty2678
@sukanyachakraborty2678 3 года назад
Brilliant explanation 💚
@mdkawser5373
@mdkawser5373 4 года назад
Best explanation. Thank you
@shreyakatiyar9041
@shreyakatiyar9041 3 года назад
Amazing👍🏻
@anjuvenkidsaraswathy6785
@anjuvenkidsaraswathy6785 5 лет назад
Well explained ..thank you.
@Mohitthakurofficial
@Mohitthakurofficial 6 лет назад
Sir abhi tak naye videos nahin aaye. Please upload as soon as possible, I'm eagerly waiting. Please upload haematology, serology, pathology, biochemistry, mol bio techniques.
@saguasodariya5585
@saguasodariya5585 6 лет назад
You are brilliant ...Brooklyn continue. with new video
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Thanx
@sukritidhaliwal8802
@sukritidhaliwal8802 6 лет назад
Well explained!.. Thanks.
@user-wi6ri4fz9k
@user-wi6ri4fz9k 3 года назад
That was perfect ,tnx so much
@Shobhi_pearl
@Shobhi_pearl 3 года назад
Explained very well 😊
@itsmejosh7045
@itsmejosh7045 4 года назад
Bro your teaching method is too good and perfect
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Most welcome Joshwa .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
@alakeshbharali5246
@alakeshbharali5246 5 лет назад
Too gooddd
@m.thishan191
@m.thishan191 3 года назад
Very nice 👍🏼
@rancysidharthan8287
@rancysidharthan8287 4 года назад
Well presented... excellent..
@alinarajpoot731
@alinarajpoot731 4 года назад
THANK YOU SO MUCH THIS VIDEO WAS REALLY REALLY HELPRFUL ...... EXCELLENT
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Most welcome
@sajidmicrobiology
@sajidmicrobiology 4 года назад
If you like our videos, Please donate us to keep work going on. You small help (starting form Rs. 5) is valuable for us. Paytm number 8830061003
@YourAhmad
@YourAhmad 6 лет назад
thank u so much.... you did a great job...😊
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Most welcome
@simransarkar4502
@simransarkar4502 3 года назад
Why are we adding the acid when we already getting the color by substrate addition only
@sajidmicrobiology
@sajidmicrobiology 3 года назад
To stop the reaction
@kaustubhrege969
@kaustubhrege969 3 года назад
Thanks for the guidance
@vatsala1902
@vatsala1902 6 лет назад
Thanku so much. Explanation is too good 😊
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Vatsala sharma most welcome
@silentclaw8011
@silentclaw8011 6 лет назад
Awesome
@abelurbina2003
@abelurbina2003 3 года назад
Sir the chromagen color dissolve inside the glass or produces colored light? Thanks
@sajidmicrobiology
@sajidmicrobiology 3 года назад
It dissolves
@jadumonigogoi845
@jadumonigogoi845 5 лет назад
has secondary antibody gain antigen property after binding enzyme to bind primary antibody!!
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Every molecule including antibody or enzyme can act as antigen when injected to lab animal. If we inject our antibodies to mice.. it will act as antigen
@keepmovingupendra4691
@keepmovingupendra4691 4 года назад
Excellent explanation with diagrams
@binbinky78
@binbinky78 5 лет назад
Excellent explanation, thank you 👍😀
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Thanx
@poonamjhamb5807
@poonamjhamb5807 3 года назад
Very very helpful video 👏👏👏
@bappabera2465
@bappabera2465 5 лет назад
Just woaw
@samikshyaacharya1032
@samikshyaacharya1032 5 лет назад
Thank you so much😊
@p.j.shruthi7198
@p.j.shruthi7198 2 года назад
Very well explained.
@rathodlal1385
@rathodlal1385 5 лет назад
Initially from where we have to take antigen...? Which are attaching to microplate surface.
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Yes
@rathodlal1385
@rathodlal1385 5 лет назад
Ur not understood my question. Ooo antigen kidarse aya
@rathodlal1385
@rathodlal1385 5 лет назад
Artificially availability is there of antigen
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Agar apko koi antibody detect karni hain to apke pass uss antibody ka specific antigen purified form me chahiye..
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Agar koi investigation me anti hiv antibodies detect karne ho. to investigators ke pass hiv antigens hote hain jo invitro synthesize kiye jate hain ya fir kisi source se purify kiye jate hain... kits main ye antigens company provide karti hain
@meeromeer8812
@meeromeer8812 Год назад
Thanks a lot.
@lakshmankaranam
@lakshmankaranam 4 года назад
Is it 4 generation or third
@jojo-my8bu
@jojo-my8bu 4 года назад
Really well explained
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Thanx
@bossyhesham6423
@bossyhesham6423 4 года назад
thanks alot ❤
@luiff9884
@luiff9884 5 лет назад
Very nice explanation sir thanks
@medzoklife
@medzoklife 5 лет назад
I subscribed.
@robertjamestaylor9261
@robertjamestaylor9261 4 года назад
best explanation
@aminuyunusa40
@aminuyunusa40 4 года назад
Very nice indirect Elisa lecture
@sakshichand1126
@sakshichand1126 2 года назад
Sir will you plz conduct lecture on how to take notes from textbooks
@aniruddh-chakravorty
@aniruddh-chakravorty 4 года назад
Where is video of direct elsiaa?
@dr.devendrabhardwaj1836
@dr.devendrabhardwaj1836 5 лет назад
Very good explanation
@Smritysays
@Smritysays Год назад
Thank you for this
@hasenmothi8752
@hasenmothi8752 5 лет назад
Sir update more videos. It is very useful to understand with animation.
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Sure
@rimalshahid3141
@rimalshahid3141 Год назад
Great
@leenusn
@leenusn 5 лет назад
Best ever
@achintyasasmal4471
@achintyasasmal4471 3 года назад
💞👍💞
@surachanarai4064
@surachanarai4064 6 лет назад
very nicely explained.. thank u sir.
@sajidmicrobiology
@sajidmicrobiology 6 лет назад
Most welcome
@shomeshali5675
@shomeshali5675 5 лет назад
Nice
@vijayachandrakalyani779
@vijayachandrakalyani779 4 года назад
Very well explained. Tq sir
@sajidmicrobiology
@sajidmicrobiology 4 года назад
Most welcome vijayachandra .. would you like to help us by donating small amount to our channel? This will motivate us to make more free videos.
@sohadalawer5808
@sohadalawer5808 3 года назад
Thank u so much
@forumedicos5202
@forumedicos5202 5 лет назад
Tysm sir
@abh3835
@abh3835 Год назад
Good explanation thanks
@jyotisingh878
@jyotisingh878 6 лет назад
very nice video sir thanks 👌👌👌☺️☺️
@rst6723
@rst6723 4 года назад
Good explanation
@mayankmehendiratta999
@mayankmehendiratta999 4 года назад
difference between sandwich anf this one
@karripradeepkumar911
@karripradeepkumar911 3 года назад
Tqq so much
@ImranAhmad-df3mi
@ImranAhmad-df3mi 5 лет назад
Excellent thanks so much 😘 sir
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Most welcome
@ImranAhmad-df3mi
@ImranAhmad-df3mi 5 лет назад
Nyc sir
@kritikajaiswal4945
@kritikajaiswal4945 4 года назад
Thank you ☺☺
@tarteelmohammed3257
@tarteelmohammed3257 2 года назад
Thank you
@yasmineyasmine6010
@yasmineyasmine6010 Год назад
thank you so much this was very helpful 🤍🤍
@sajidmicrobiology
@sajidmicrobiology Год назад
Most welcome
@afrozaga4464
@afrozaga4464 5 лет назад
Direct also plzz sir
@sajidmicrobiology
@sajidmicrobiology 5 лет назад
Sure
@afrozaga4464
@afrozaga4464 5 лет назад
@@sajidmicrobiology thankyou so much sir.
@meghanac6128
@meghanac6128 3 года назад
Awesome
@rishishet3330
@rishishet3330 4 года назад
Awesome
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