That's pretty clear information but I don't understand the content. This is where I love science but don't always understand it. Then I get frustrated and zone out. It's really not a criticism to the presenter at all. It's knowing myself enough to know I don't understand. With science I know I am much better at the questioning than the answering. I know in science I need very practical everyday examples to understand things.
Every one Read this please. Doubt clear Ion exchangers are either cation exchangers, which exchange positively charged ions (cations), or anion exchangers, which exchange negatively charged ions (anions). There are also amphoteric exchangers that are able to exchange both cations and anions simultaneously. The resins consist of aggregations of big insoluble molecules carrying fixed negative charges, which loosely bind positively charged ions (cations); these readily exchange with cations in the fluid environment to an extent that depends on their affinity for the resin and their concentration. In cation exchange chromatography, the stationary bed has an ionically negative (-) charged surface while the sample ions are of positive (+) charge. This technique is used almost exclusively with ionic or ionizable samples.
Quick biochemistry basics is my favorite channel. by the inspiration of this channel i started my channel covering all topics regarding pharmaceutical analysis & biochemistry. i did video on ion exchange chromatography
thank you for your effort . but l have a notice for you, My friend, Cation Exchanger have a positive charge,while Anion exchanger have a negative charge. thank you again for you .
Not true. The exchanger itself has the charge opposite that of the ion that is to be bound. If you want to bind a cation to the exchanger, it need to be negatively charged.
bro you are correct that cation is positive charge but in case of ion exchange if we have cation exchanger. it will have negative charge on it and will attract positive charge and this will be able to exchange the cations and hence it is termed as cation exchanger. here the name and charge are of opposite nature cation exchanger - negative charge anion exchanger - had positive charge on it.
Great video! I will be looking for a video of yours with greater detail of what happens to the analytes/proteins when exposed to a pH gradient during ion exchange chromatography. Thanks!
Nothing simple about this for sure. Can you make a short one explaining for lay people. Our local water plant just switched to this type of water cleaning and I'd like to know if it means they'll use less chlorine now.
Then you need to look for a video explaining ion exchange. Ion exchange Chromatography is a system solely for detecting substances such as proteins. The system of ion exchange (without chromatography in the name) is for filtering. But I don't know anything about water filtering so I can't help you :/
