Recording of Lecture 6 of "Biological X-Ray Crystallography and Cryo-Electron Microscopy (C7270)" course. This is the second lecture about cryo-electron microscopy covering: Spatial waves, Fourier transform, image formation and
contrast transfer function (CTF).
00:20 Spatial waves
12:40 Fourier decomposition
21:41 Fourier transform
30:05 Reciprocal space
41:22 Fourier transform of 1D discrete waves
47:50 2D spatial waves
56:57 Fourier transform of 2D discrete waves
01:03:10 2D reciprocal space
01:08:00 3D spatial waves
01:14:26 Fourier transform of 3D discrete waves
01:17:19 3D reconstruction
01:23:41 Reciprocal space - properties, examples
01:42:52 Convolution
01:49:15 Electron scattering
01:53:02 Image formation in TEM
02:02:26 Principles of phase contrast in TEM
02:11:26 Contrast transfer function (CTF)
02:17:06 Envelope function
02:19:01 Point spread function
02:24:07 CTF correction
02:29:44 CTF estimation
02:34:25 Questions and answers
18 сен 2024
