Keeping a running tally of how many of each mushroom you should have at the end of each chapter. After Prologue: 2 Spotted Crown After Chapter 1: 8 Spotted Crown, 7 Sandy Needling, 1 Cupling After Chapter 2: 19 Spotted Crown, 8 Sandy Needling, 9 Cupling, 4 Druffle
I'm pretty sure i know the one you're talking about as it was my last 1. There is one in the first area. After you break the red barrier you will need to posses an armadillo to ger under a rock. When you are on the opposite side use your vision and you wont see a yellow spot but a black spot for some reason. That should be your last 1 hopefully.