Making and Testing The ANTI-SPICY Molecule (Capsazepine) 

There is a much simpler chemical that selectively blocks TRPV1. en.wikipedia.org/wiki/AMG-9810 You could make this really really easily. It also probably tastes better since it has a more neutral pH and it doesn’t trigger noxious chemical receptors.

tasting your own chemical product...... That's either really stupid or fucking savage!

Getting rid of the burn and the taste is no help. The spice is still going to do the damage, also you are still going to feel the burn on its way out😱💥💩

I believe I am the man your looking to prove this. Perhaps I can prove you right!

Been breeding Ghost Peppers for 14 years, milk and bread are placebo. The real trick is honey.

Is it possible that it's like capsaicin and needs to be in a salt form to be effective? Or was it already prepared injestion? (When i say salt form, something that will disolve in water as pire capscaicin doesn't have a strong effect until it dissolves slowly).

@@nunyabisnass1141 I was told, alcohol latches onto the capsaicin even better. Taking a shot of tequila and swirling it around in your mouth after eating something spicy would flush it out. it WILL HURT a lot more than the spice, but it's for a shorter period, because the capsaicing is flushed away and doesn't dislodges from the receptors

@@iWhacko i have no idea, I know that it's more soluble in alcohol, but that's as far as my knowledge goes.

Did you take it before or after you ate the spicy food?

Please elaborate in detail.

​@@ryanjohnson4565🍑🔥🧯

I assume it would also affect nerves elsewhere if you swallowed it...

@@TheHuntermj I suppose that depends on the dosage, and how well it survives stomach acid and enzymes... The mouth is a far less harsh environment, chemically, than the rest of the digestive tract.

Well, amateur doesn’t really denote a lack of skill, but rather an independent chemist who works outside of a laboratory/company. It is, however, a very impressive reaction route.

​@@chessgaming9942I think you've passed "amateur" in chemistry when you can look at a molecular chain and go "yeah, that'll explode if exposed to oxygen."

He isn't scared of the byproducts, he IS the byproduct.

It's a very small amount, but still... it's always a risk.

"it's more convenient to purify it later" purification later: wash it with some water and into the mouth it go

The problem with home chemistry labs. No money for test equipment. Some things just take a University.

@@guygordon2780 apparently not this thing, though, since he got it done

IRTX and capsazepine are both antagonists of TRPV1 which means they block activation of the receptor. You might be talking about inverse agonism, but I don’t think IRTX is an inverse agonist of TRPV1 and I also don’t think inverse agonism if TRPV1 would give a cooling effect. Like you said, menthol acts via a different receptor to produce to cooling sensation. What makes IRTX so interesting is that the non-halogenated RTX is an extremely potent agonist of TRPV1 (1000 times stronger than capsaicin), and adding that single iodine completely eliminates the molecule’s ability to activate the receptor

@@pente12 yeah it is interesting isn't it. Just like in the most crude visual sense, estrogen and testosterone are vaguely similar looking molecules. And you can take a very potent traditional opioid, oxymorphone, and just at a single allyl group to the nitrogen and suddenly you have a potent antagonist naloxone.

@thomasrussell4674 Yup, pharmacology is wicked cool. There are also opioid agonists and antagonists that covalently bind to opioid receptors, so they basically work for days until the receptors themselves are broken down. They’ve done a lot of pharmacological research into different opioidergic compounds, so that entire class of drugs is very intriguing to learn about.

@@Sniperboy5551 yes you're exactly right and the only covalent bonding example I know is oxymorphone itself. I'd like to know if fentanyl or etorphine is capable of this additional form of receptor bonding. It's funny the way some old fashioned super strong opioids like levorphanol and oxycodone were actually quite long acting, while some modern high potency synthetics are ultra short acting. Interestingly the covalent bonding rapidly accelerates the formation of tolerance, because nearly all opioids cause beta arrestin recruitment and lead to receptor internalisation, but normally this requires repeated and frequent dosing to trigger the cell/the body (which seeks homeostasis and reacts to continual over activation of an opioid receptor as unusual and something to be stopped) to effectively put the overstimulated receptor out of commission. Actually homeostasis works in at least 3 ways to counteract ongoing opioid intake, 1. Plasma antibodies to bind and inactivate an exogenous opioid before it gets to a receptor, 2. Internalisation 3. Eventual upregulation of substance P to maintain pain perception and gut motility and 4. Something to do with expectation, I can't remember the exact mechanism but there's a weird counterintuitive nocebo effect which can be blocked with proglumide, but only if the person was expecting a drug dose, basically the body tries to prepare itself if the person is in a state of anticipation and it secretes a type of neurokinin, but proglumide can block it. Only 2 opioids I know don't cause beta arrestin recruitment. 1 is a strange, non-nitrogenous relative of salvinorin but it's not a mu agonist, I think it might be delta. 2 was a big pharma designer opioid called oliceridine which was designed not to, supposedly to be less addictive....But it didn't make it through multistage clinical trials. There's another fascinating old drug

Sorry it was meant to say levorphanol and oxymorphone , but it all ends up the same because of cyp2d6

Hey it’s the other chemistry guy

Ah, we've all done stupid shit in a lab lol. I once rinsed out a Buchner funnel with water, forgetting that it had been used to filter out sodium hydride. The pyrotechnic display was not appreciated.

This was my own youthful experience. Now, 4 decades later, about 6-18 hours after my spicy meal my family members stand in the hall outside my locked bathroom door asking why I keep yelling, “Come on ice cream!”

@@STEAMerBear I had that for a few months, but in time, both ends grew accustomed to spice and now it isn't even a thing. Just flavor really.

@@thricegreat7175 Very much the truth. Try it for yourself. throw some cayenne pepper powder on your tongue then take a swig of whole milk. Burn disappears. Repeat but with water, burn just gets worse.

Yeah, I'm a HEAVY milk drinker and can 100% confirm this is not true. If does help, like other fats, but nah, it's a far, far cry from "cancelling the spicy."

@@TravisTerrell I'm gonna listen to my own tastebuds over a rando on a youtube comment section 🤷 and my tastebuds confirm it to be right on the money.

"We saw NileRed have multiple failed attempts at the synthesis of benzaldehyde... likely due to some small mistakes that ended up ruining the whole experiment; which is quite typical for chemistry, and there is some art in finding and fixing them which often requires advanced knowledge of chemistry, ACQUIRED THROUGH MANY YEARS OF SUFFERING" - Chemiolis Focus on how rewarding it will be to be able to do things the majority of humans can't even comprehend! And when you apply for a job, you'll get way more money if you search for "material manufacturing" instead of "chemistry or lab work" Like 100k+ manufacturing coatings for anything from lubricating gears to coating spaceships for NASA or SpaceX

Talk to the university’s welfare department. It’s what it’s there for and they may be able to help. They are used to burnout and should have procedures in place. Don’t be put off and nag them until you get a result.

Just at the end of my 1st semester, 2nd year of studying chemistry of materials and chemical engineering. Am not that far as you, obviously, but have little to no motivation to do anything. I think the only motivation I have is that I like learning chemistry (while also hating it, insane, right? xd). I just look at some people and want to do the work they do and to do that I need to finish at least the Bc. But I would literally trade the ability to make PCHEM disappear instead of world peace, ngl.

Life is a marathon, it will be OK bro 🫡

Everything worth doing is difficult. The coolest shit has tough moments, otherwise - everyone would be doing it. If you are continuously over- burdened, burnout is imminent. Think how could you lessen the load - can you share the work differently? Move somewhere closer or change hours you have to be physically present at the lab so the commute is taking less time? Could you talk with department to swap some hours? Perhaps the shorter days and lessening amount of daylight is affecting your mood. Think of how you can incorporate daylight exposure - a short,brisk walk a day helped my mood and energy levels tremendously. You can always use this time to listen to lectures or audio notes, but I think it's more valuable to unwind. Have you thought about utilising some AI tools to lessen the workload? It's not a sin to use AI to redact lab notes, or rephrase papers or notes but I've heard it can cut out some of the tedious aspects of work. Remember, the uni department is there for you - contact them if you are struggling. Asking for help is an important skill. Remember that the boring parts are a part of a test and as all troubles - are temporary. Don't make same mistake I did and run yourself so far into ground with pointless perfectionism that there's no way out but to give up.

It looks like he bought it from Sigma Aldrich. The bottle looks like a Sigma SureSeal bottle.

I love it. Peppers especially fresh, have some of the most complex flavor profiles of any flower. This is especially true for the super-hots. I eat Carolina Reaper powder in my soups and with chicken noodle soup, you can't pick a better spice to pair with a nice yellow curry.

Can't wait for the capsazepine bidet to be installed at chipotle

Trust the process 😂

i'm also curious

Well, the high-field NMR in my basement isn't working right now. :-)

@@guygordon2780 No need, we can just guess the resultant spectra :)

Unless you work at a university, you don't have access to those

@@durshurrikun150 For a fee you can get those done as a service at most universities. In fact, you can often rent labspace and facilities. It beats harming yourself for a youtube video.

@@user-zx8oy2jf4d Yes, but it costs, it's not free.

That'll make a good Chubbyemu series!

LiOH is more soluble in organic solvents then NaOH

I like that capzaicin cream (the brand is spelt with a z) , anyway the packaging looks exactly like the cortazone and my girlfriend used it on poison ivy blisters!!!😵😵😵😵😡😠 She wasn't happy 🤬

@@petevenuti7355 On the bright side, bet she forgot about the sting from the poison ivy

Did it actually help with the pain?

Sounds like some kind of gymnemic acid. It is derived from a plant known in Hindi as _gurmar_ which means sugar destroyer.

Try chewing up ibuprofen pills. You'll get a similar effect

@@trippyvortexit's the opposite. people use it to lose weight, especially those with diabetes, as it makes it less desirable to eat the sweets.

beetus

​@@trippyvortexyou either have pica or misread the comment 😅

Ginger contains gingerol, which is chemical that also activates trpv1 like how capsaicin does. I assume it would either activate the receptor weaker than capsaicin and compete with the capsaicin trying to bind with the receptor, or it might desensitise the receptor making the action of capsaicin weaker.

I think this does the opposite: makes you not taste the spicyness, but it’d still destroy your guts and third eye

​@@kaleoscreations8069 The worst of both worlds.

Although not as serious as yours. My gut also can't tolerate too much capsaicin. My tongue/mouth are enjoying capsaicin. The heavier the dose the better. But i will have to go to the toilet multiple times when i got too much of it

"Is it safe to be near this stuff while you're making it?" The answer depends on what exactly you mean by that. Nothing here is radioactive, it's not gonna kill you just by being somewhere near you. But in general chemistry can be dangerous, that's why you always follow some basic safety protocols like wearing safety goggles, not getting food near your chemicals, doing most things in a fume hood (which protects you from nasty gasses and also other dangers like splashing or light explosions), etc. You have to respect the chemicals, they can burn you, poison you, explode, etc. If you have a more specific question, I'd be happy to provide more details. "How do the separatory funnel work?" The piece of glass itself doesn't really do much except hold the liquids. You put in two immiscible (not mixable) liquids with different densities, shake them around so they get in contact with eachother (during this substances can transfer from one liquid to the other depending on their solubility), take care to vent regularly so no pressure builds up and then you just let it sit while the two liquid layers separate due to their different densities. Then you can drain them out one after the other. "How much does this setup cost and is it possible for a regular pleb to just look up stuff and start synthesising things for fun?" Good glassware is generally not cheap and chemicals can cost quite a lot. Also you definitly need a fume hood and I can't imagine those are cheap to acquire and install. But I'm more concerned about your safety. I don't feel comfortable with the idea of somebody who has no lab experience trying to do organic synthesis unsupervised at their home. You don't really know what's dangerous, how dangerous it is and how to properly protect yourself. And that starts with basic things like properly storing everything.

@@lunkel8108 you're a legend! Thank you for taking the time to respond to me. Yea.. I got a bit too excited as it was the first time I saw something like this and it seemed to cool :D I then quickly realised it's not a good idea to attempt this stuff on my own and I'll just continue on my software engineering path. Ty again for the reply! I learned quite a bit

Isn't that the compound that blocks bitter tastes? Appears in a fruit as far as I know.

@@AllisterCaine It makes sour taste sweet.

@@WaffleStaffel ah, that's what I was thinking of. Remembered it wrong it seems. Some company sells this stuff as a gimmick in chewing tablets.

@@AllisterCaine It's a really fun sensation. It makes lemons taste sweet, makes pickles taste like sweet pickles, vinegar taste like sugar water, etc., and it can be used as a non-nutrative sweetener for diabetics and calorie reduction. Big-pharma controlled FDA has failed to approve it for this multiple times, even though it's just a freeze dried fruit. Too much money at stake.