Hi! Just wanted to say thank you for making the video!! It was really thorough and easy to follow, and really helped me out with my science fair project. :)
I think 15 minutes is the standard using a pressure cooker/autoclave. Regular boiling (not pressured) takes 40 minutes. Thanks for the info! Btw... I wish you'd say "sterile" versus "clean." There's a real difference.
I'm surprised you're not using a still air box! I'm learning how to grow Shiitake mushroom and I'm starting from a Liquid Culture. My first two attempts were completely a bust, but then the next two were perfect and have clean mycelium on them. So I'm a bit excited for that!
@@brightbiology9632 I think they are talking about sterile transfer box, not an incubator. Im curious as to why you did not have a bunsen burner or alcohol burner going to help keep a," cone of sterility" as i have seen in other videos and books? Any reason, or is it not needed when just making plates?
This is really helpful! Question though, when I was researching about the ingredients I needed it said to get “Dry Malt Powder” but I’m not sure what that is used for or how much I need. Also would using “Agar Agar powder” work the same as what you’re using?
Agar agar powder won't contain nutrients required to support culture growth, but you can create nutrient agar powder using agar agar powder. You can probably find a recipe online, but I imagine it'll be some parts agar agar powder and some parts potato starch or malt powder or sugar.
Hi..What is the actual Full recipe for making the liquid agar please..as I don’t see the amounts tht used..( I may hv missed them cause I’m disabled) but I would b v happy to know thanks xxx
Hello, it's a helpful video! I have one question about the water though. Can I use water from the tap, or is it a must to make agar with distilled or purified water? Thank you for your time!
Actually yes you could do this. there's a channel called 90 second mycology (which has more to do with Mushrooms but the guy covers some agar stuff which is pretty neat). Just look up no pour agar on that channel. pretty ingenius.
@@brightbiology9632 Thank you! I guess I could give it a try, seems more sterile in my conditions without a laminar flowhood. Or maybe a alcohol lamp would do the trick...
@@jerseyhovidea use a still air box buddy… or hear me out, get the 4oz mason jars and they won’t boil over if you go just under the agar line with the water line
They are just different types of plates made from different materials. Many times people will use different types of plates to isolate various bacteria or identify them.
PDA----Potatoe Dextrose Agar. MEA-Malt Extract Agar. all use agar for the jelling, but different nutrients. Depends on wht you want to grow. The nutrients are the food for the bacteria, or fungus or wahtever it is you want to grow. Different things like tt eat different stuff.
Final someone to put “Dr. Edward Grand” in his place he’s supposedly gone to college for at least 8 years in his own words yet he still acts like a psychopath when you ask him about something he doesn’t know about.
Why can't we just do the bacteria experiment on the sterile petri dish? Why do we need this agar stuff? I want to check my neighborhood bar for bacteria, cleanliness - offer to clean and disinfect them on weekend nights.
The agar allows you to grow the bacteria, it acts as a growth environment and food source for the bacteria. If you just had a plain, empty dish, nothing would happen.
