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Microscopy: Phase, Polarization, and DIC (Stephen Ross) 

iBiology Techniques
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Learn more: www.ibiology.org/talks/dic-mi...
This lab illustrates the components in the optical light path and how they need to be aligned to achieve proper 1) phase microscopy, 2) polarization microscopy and 3) differential interference microscopy.

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10 ноя 2013

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Комментарии : 16   
@Dhelira
@Dhelira 4 года назад
Very enlightening. Thank you
@AMadScientist
@AMadScientist 3 года назад
Hello. Thanks for explaining. What I'm trying to find out is how a DIC scope would view live protists? I see videos on RU-vid with these great clips using a DIC but I was wonder if it is just "drop the water on the slide and go" or is there tons of prep time to get the "good" shots. Thanks
@HaloGrndr
@HaloGrndr 4 года назад
Any chance you could provide some part numbers for the polarizer/analyzer components? I can't find anything!
@source1289
@source1289 6 лет назад
was the lower prism left in for phase contrast ?
@ClownWhisper
@ClownWhisper 5 лет назад
I just got an older TSM inverted microscope and I was wondering is there a camera mount or a camera made that specifically fits the neck of this microscope? I have seen what I thought was a TSM equipped with a completely different neck with a camera going straight up and the regular binocular assembly in its usual position. I have no idea what I should look for can you please help me?
@Thesignalpath
@Thesignalpath 3 года назад
Thank you.
@johndubose6589
@johndubose6589 Год назад
Thankyou!
@Satchmoeddie
@Satchmoeddie 9 лет назад
Where can I rent one of these and all the goodies for 2 hours? Amazing scope! Nikon, Canon, Leica, all make nice stuff. I need to do some color phase shift work on 4 samples. That is a great unit! How many human kidneys does it cost?
@abskateboard
@abskateboard 5 лет назад
not to mention zeiss lol
@JakeSchonhoft
@JakeSchonhoft 4 года назад
Can't forget Olympus. Been making microscopes since 1919.
@donsimons9810
@donsimons9810 3 года назад
color phase shift work? not to dork up the lingo, but what do you mean by that?
@MrGunzoller
@MrGunzoller 3 года назад
Why use expensive DIC method while you can just use oblique lighting and to see absolutely the same 3D effect? You don't even need a specific instruments, just ordinary biological light microscope. All you need is to play with condenser diaphragm (about 50% closed), opaque screen in front that diaphragm and light volume.
@donsimons9810
@donsimons9810 3 года назад
do you have a reference for this technique used in microscopy?
@MrGunzoller
@MrGunzoller 3 года назад
@@donsimons9810 ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-ocTEV6JOmVc.html
@FieryCoal
@FieryCoal 2 года назад
Although Oblique is useful for its cost, it gives off a lower image quality than DIC.
@abedhossini5793
@abedhossini5793 7 лет назад
boring not sobject yor act
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