Microscopy: Resolution in Microscopy (Jeff Lichtman)

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This lecture describes the diffraction of light, a key principle in image formation and a factor that limits the resolution of a conventional light microscope. The behavior of light traveling through an objective is described along with the concept of numerical aperture. The "point spread function" (or PSF) and Nyquist sampling are explained, which are critical concepts for understanding image resolution and detection of images.

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10 ноя 2013

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Комментарии : 20

@kenschwarz8057 2 месяца назад

What a revelation! That Huygens figured out the fundamentals in 1690 is also mind-blowing. Thank you for this fascinating presentation. It utterly changed the way I think about light and optics.

@stuartgreene5010 4 года назад

I've been searching for a clear, conceptual explanation of how and why optical resolution depends on the diameter/NA of a lens. Explaining it in terms of wavelets and illustrating the incremental influence of adding wavelets from larger arcs of the initial spherical wavefront hits the nail on the head. I finally understand this in a way I can explain to others...and also, of course, to show them this video! Great job.

@davesantiago1827 Год назад

You should also explain this is the reason we see things disappear bottom up and not due to earth curvature like claimed. This proves they have hijacked perspective and call it earth curve. Earth is flat. Please don't dismiss me because of your belief in the globe. Follow the actual science like this and make an objective opinion.

@BuysDB 9 лет назад

Great presentation! I really like the way the model for the viewer is gradually expanded during the story, keeping required prior knowledge to a minimum and allowing for fast understanding of the basics in this topic. Thanks.

@OOPECHO 9 лет назад

BuysDB DB cant agree more, excellent presentation!

@hussainali9999 8 дней назад

Thanks for sharing your knowledge.

@yasminali7578 6 лет назад

well explained and simple.... this lets us distinguish people who understand the material they are presenting from those who dont.

@mikkokylmanen9296 7 лет назад

Very clearly explained, thank you!

@Dhelira 4 года назад

Great lecture. Thank you, sir.

@benvos2458 5 лет назад

I'd just like to point out that the name Lichtman means Lightman in Dutch.

@MiottoGuilherme 2 года назад

I'd just like to point out that in German too.

@Livecellimagingfacility 9 месяцев назад

The equation 0.61Lambda/NA is sometimes presented as the radius of the main lobe and sometimes as the diameter. It is the radius. :)

@frankied.2828 5 месяцев назад

Thank you sir, for your tremendous videos.

@xyandz1913 4 года назад

Thank you, Sir!

@dc74835 5 лет назад

Thanks for making this video.

@yoanagergova1894 4 года назад

Thank you for the great video! I would like to ask what I should use as lambda in the formel for the limit resolution by epiflurescence. d=0,61.lambda/ NA What is lambda by epiflurescence: excitatationwavelenght or emissionwavelengt. Or is it something with the stokes shift?