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Migration Assay 

Abnova
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( www.abnova.com ) - The migration assay (also known as the Boyden Chamber Assay) is a commonly used test to study the migratory response of endothelial cells. During this assay, cells are placed on the upper layer of a chamber and a solution containing the test agent is placed below the chamber. Following an incubation period, the cells migrated through the membrane are stained and counted. More videos at Abnova www.abnova.com

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30 сен 2024

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Комментарии : 24   
@asmaaamel1840
@asmaaamel1840 10 лет назад
you wash with PBS+ or PBS - ? how much volume of giemsa, methanol and formaldhyde is added ? 3,7 % of formaldhyde in PBS- or PBS + ? it should be noted
@muthiikawati8306
@muthiikawati8306 10 лет назад
I have never been using this method, but as far I know Boyden chamber has a porous membrane at the bottom where migrated cells "trapped", scrapping only remove non-migrated cells. cmiiw
@rippeduthjghgkj9
@rippeduthjghgkj9 9 лет назад
did anyone count?
@Abiha-ASM
@Abiha-ASM Год назад
After swabing with cotton how do you know which are migrated cells??why cotton swapping done???plz assist
@calummcfetridge7649
@calummcfetridge7649 2 года назад
Most protocols I've seen suggest scraping off non-migrated cells prior to fixation and staining. However I can see the appeal of performing this step after staining, as you can see if the apical stained cells have been removed. Does anyone know if either method is superior?
@takina9445
@takina9445 Год назад
Do you mean scratch by scraping?
@mitalbhatt5558
@mitalbhatt5558 7 лет назад
how to cite this video tutorial
@Throughmylens01
@Throughmylens01 Год назад
what is the catalog number of the migration chamber?
@francescosilvestro2092
@francescosilvestro2092 2 года назад
Dear Abnova, where could I found the music in the background? Are there any kinds of playlist?
@learn-bioinformatics
@learn-bioinformatics 10 лет назад
AWESOME!! It really helped me a lot!!
@kunalapavan
@kunalapavan 13 лет назад
Thanks for sharing. It will be very helpful for me while performing the experiment.
@abdullahimohamedsaid1813
@abdullahimohamedsaid1813 5 лет назад
is it same procedure when we use crystal violet die? this experiment is used Giemsa stain instead of crystal violet.
@yannacademy
@yannacademy 6 лет назад
Thanks for the video. I especially appreciate the comment that follow the video indicating the layer that contain the test agent. Thanks a bunch
@20rayt
@20rayt 5 лет назад
It would be good if you cut the membrane and then mount it on a slide and observe/count cells. Thanks
@prayaschakmashanto5275
@prayaschakmashanto5275 2 года назад
How much pore size of the insert?
@deborahwokpetah6600
@deborahwokpetah6600 7 лет назад
THIS VIDEO HELPED A TON!! THANK YOU
@iraklismoschonas5214
@iraklismoschonas5214 7 лет назад
Do you know how I can count the migrated distance of the cells?
@hamadali3400
@hamadali3400 6 лет назад
Iraklis Moschonas I think some researches using image J software
@manuelp.jimenezgarcia6703
@manuelp.jimenezgarcia6703 10 лет назад
Does anyone have the exact reference for Abnova's boyden chamber product?
@魏欢欢
@魏欢欢 Год назад
Thanks!
@ShanatSD
@ShanatSD 12 лет назад
Thanks for the video, it was very very helpful!
@judnarokjudnaddff6541
@judnarokjudnaddff6541 12 лет назад
excellent vidio.
@m7mady0
@m7mady0 11 лет назад
great video
@pallekranthi
@pallekranthi 11 лет назад
excellent
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