Mutation Predictions: Identification & Naming of Mutation by BlastX: Lec 9 Part1 Dr. Muhammad Naveed 

I wish I understood more of what you are saying. As it is, I only caught an English word here or there. Nevertheless, your video helped me a lot. Thank you so much! Indeed, I found it more helpful than the NCBI’s tutorials (which are great). I like how you got straight to the point and demonstrated the best way to get the comparison done. I feel more comfortable using Blast now. I am wondering whether there is way to take the analysis one step further. Let’s say you blast a sequence and find a similar sequence in the database. Then you select “Pairwise with dots for identities” and find a variant in red, like in your video. Is there a way to easily connect that variant directly to dbSNP or the ClinVar database? Anyway, thank you for making this video. It is superb.

Kis cheez ko improve kardu ju mujy Ms main help karday

In case nucleotide sequence -G seen in the subject,against my query sequence

Sir g main Uk jaraha hu Ms k liya Us k hwalay s turee discussion karne the

Sir,humne blast x hi q use kia hai yaha pr? Hum direct protein ki fasta seq ko lekar b blast kr sktey hai?

Sir ...why we are identifying mutations using protein sequences ...why not using nucleotide sequences ..~~~ Please reply me Sir ..

Hy sir I just saw your video related to bioinformatics I got assignment related to it I need a help because my teacher say I have to introduce a mutation but I don’t know how to do it if the match is 100% in blast software I can’t do it please help me related to that point

Sir Best thing is that you answer students' questions in comments that is great quality, our country need such type of responsible persons

if i have protein sequence... for mutation identification then i have to convert protein sequence into nucleotide and then i will use blastx for mutation? please tell for protein sequence

you are saying that we perform Blast X for DNA to Protein can we also go with protein to protein blast for the mutation prediction

Good luck...Keep it up👍👍👍👍👍👍👍Hero of UCP❣️

great participation of Dr.Naveed would be considered in Pakistan in future, thank you Sir,

Asslamualikum sir i am working on protien which is cancer causing.For sequence i take gene sequence or protiene sequence? because i have to analyse mutation in gene.

Sir how it will be check whether the predicted mutations are novel or not?

Can you tell me how to pinpoit snp in bioedit using abi format??

Sir ji mujhe ek chez samajh m nhi aa raha h ,ki hum Blastx m nucleotide se Protein m translate karte ..hai and Jo new page m results aata h usme hum ko alignments ko select karke mutation identification karna hai ..but instead of blastx ...if mere pass protein sequence h already then can I choose pBlast or Blastp ~? Qki usme vi ta same result aayega na ...then aur ek doubt ye hai ki ...hum mutation analysis kis sequence ko karte hai ? Jis sequence ko as a reference lete hai ? Or Jo alga alga organism's m us protein sequence ka homology milta hai ? Please reply me .. Thank You

Thank you so much for such an informative and helpful video. I have followed the same procedure for actylcholinestrase, but I didn't find any mutation. I have found A4T at 11 position and T4L at 6 position, but I failed to find it on pair wise with dots for identities. Would you have some time to help me out. I will be highly thankful.

Keep doing the great work, Sir. I'm very impressed. Still waiting for our English Version. 👍👍👍

Maza aa gya... Jnb

Sir can we put the fasta sequence of snp like rs742935 to check the blast or to name the mutation

How to verify mutations in sequence ?

A.o.a sir ... I am doing my work by watching your tutorials... These are amazing and well explained but I have some queries can I contact you sir??? Can you please share your email or contact with me ... It's really urgent

Sir it will be very helpful if starts making lectures on, how a student uses bioinformatics to find identifies and validate a risk SNP or enhancers for his experimentation.

Thank you sir for your details information.... 🙏

I want to predict mutation analysis for a gene through SNP of the blast but I want to know how to download the SNP of a gene from the NCBI and then upload that to the SIFT kindly guide me sir

I understand very will mutation identification....

Plz upload lectures on docking

Nice lecture.Sir i have a query suppose i want to check mutational frequency of a protein ,such as SARS-2 spike protein.Mutational frequency means suppose i took 100 Spike protein sequence and convert them into gene,Now i want to check the mutation point among this 100 gene of that spike protein(that means how much positions become mutated).Moreover i want to see which positons become more mutated among this 100 gene.That means i want to know the frequency of particular positons.Kindly help on this

Sir plz uplaod the video on pymol tool for target the muted region in structure of any protein.

Sir please thoda zaldi video banaye amino acid substitution matrix par

Sir kya ye clustalw k tool pr jaw kr hum mutation ni dekh skty?

When you upload the second part of lecture 9

Nice videos

Hello sir

💥💥💓💓🖤🖤

thank you sir , keep it up