8:07 as a Rick and Morty fan, I absolutely lost it when you said shleem. Is that actually a real word? I tried googling it but only Rick and Morty comes up 😂
I don't know when or why I subscribed to your channel, apparently I once watched your video before this about the AC, but I do not remember doing so at all. That aside, I'm glad I did, looking at your other videos I will surely watch all of them and enjoy every one. Thanks for the neat video! I have some oyster culture in a shoe box on agar, though I moved away for some time, so I wonder how it will be doing when I finally get back to it, it must be about one year since looking at it.
if you drill a 5/32" (4mm) hole in your lid the syringe filter fits perfectly & will not pop out. you will need to give it a good push to fit it in the hole & drill a clean hole.
Does plastic expand and contract in the pressure cooker though? I thought I read that even if one drills the perfect holes in one's lids, it is still advisable to set syringe filters and rubber Self-Healing Injection Ports with RTV silicone to ensure no external air gets pulled in around the hole, rather than through the filter. I could be wrong. I drilled many perfect holes in my lids and the SHIPs were incredibly snug, but I figured if they will contract too much when PCed, then I've defeated the purpose of making the lids in the first place... so I just went ahead and RTVed them all in.
So....I have been growing P. polycephalum for a while and continuing the culture. However, when I try drying it either on oats, paper, agar or card....NOTHING seems to come back to life. I have dried it multiple ways and tried several methods. Any idea what on Earth I am doing wrong?
How do I remove mold that appears on the oats? And if I want to kill/ reduce Certain part of the mold so that it is not so large how would I do that? Also how would u dispose of the slime mold when it done using a specimen and don’t want to make it dormant?
It isn't strictly necessary to sterilize them, but if you want to reduce the rate of contamination, you can load the oats into a mason jar and pressure cook them. Drill a hole in the lid of the jar and glue a patch of Tyvek (USPS envelope material) wlusing high temperature silicone. This will keep the oats from getting saturated with water from the pressure cooker while letting them get to pressure and temp. 30 minutes at 15 psi should be enough.
No they eat the oats themselves, after they finish with one, there is usually just a tiny remnant. They are generalists though, so they'll eat most bacteria as well. Myxos especially enjoy eating fungi too.