nectarines im obssessed with, dont really like peaches though, theyre too dry, nectarine are like 90% water theyre so delicious and refreshing. one of my favourite fruits
I absolutely love your videos, have had a little crush on you for years. They're so informative and you can see you enjoy what you do, keep going, Alex!
My problem with CRISPR is that I want to make, like, a feature length documentary on it! It's so cool! I have so much to say! So many people to interview! Making a single RU-vid video is proving to be challenging...
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This is glorious, I been tryin to find out about "are nectarines supposed to be soft?" for a while now, and I think this has helped. Have you ever come across - Giyaarter Unflappable Gratification - (should be on google have a look ) ? It is a good one of a kind guide for learning what to eat to balance hormones and eliminating trouble fat spots minus the normal expense. Ive heard some extraordinary things about it and my neighbour got excellent success with it.
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Wonderful, nice video. Thank you! I am always amazed that 60 years ago, we didn't even know yet how genetic information is encoded, and these days we are able to isolate pieces like here, or even can calculate how a folded protein might look like. Science is amazing.
The coolest thing about this video is There's another Woman Doing Science on the WEB! How great for little girls to see they can grow up and do cool science stuff!
In the graphic at 3:00, it states that that the nectarine-specific allele segment is "too long, no amplification." What does that mean? I've worked with PCR a few times in my current undergraduate studies, I don't recall anything about segments not replicating because they are too big. I would have expected lower amplification levels, but not none. Or were you controlling for that during the PCR?
Good catch! The PCR amplification step was pretty short, so the polymerase should not have had time to amplify such a large chunk. However, I used a slightly different polymerase than the one in the original paper, and it's actually quite fast, so you *can* see some potential faint, large bands at the very top of the nectarine lanes! But ideally the amplification step should have been too short for them to appear! There is eventually a size limit to what you can amplify with PCR, but with current enzymes it's in the tens of thousands of bases...
Another all-around great video! Intentional or not, the little tongue-slip into the up-state 1950's accent at the end was great. I can't hear the word "allele" and not picture a beautiful dame in a little black dress myself. haha They say peach-fuzz protects the fruit from rainwater and parasites, but I wonder if the hairless nectarine has another evolutionary advantage, perhaps in a different environment?
Crabgrass. Why is it that crabgrass thrives in drought, which we are experiencing in New England (your old stomping grounds) right now, but turfgrass does not? Might there be a way to introduce the drought-loving properties of crabgrass into lawn grasses like fescues and bluegrass through the magic of genetics?
That's just peachy! Verrrrry interesting stuff, especially the part about how you make the fragments visible in the gel, I have learned many things today!
I'm loving your channel! (I have found it through the "Valentine's day" video by Inés!) In particular this video is great! I have only taken a very basic introductory course in cell and molecular biology, and I have found it amazing to have it all shown in a practical example in the lab! And I believe that it is the first time that I could understand how the game of dominant and recessive gens work at the molecular (DNA) level. I would love if you could make a video explaining how PCR works in more detail (as you somehow have promised in this video!)
I would love to make a video describing PCR in more detail! It's on it's way. I have big plans for it! And I'm so glad that you enjoy the channel and are learning some things along the way!
It would be cool to talk about CAM plants or C4 plants as alternatives to C3 photosynthesis. Maybe get some pineapples (CAM plants) in there if you want to continue the fruit theme
Ok, I have one: but it's not about fruits, it's about flowers. Do you know "petunia night sky"? It's a recent (I think) cultivar and I was wondering about the genetic and molecular mechanism that makes possible this beautiful pattern in flowers. Maybe has something to do with transposons?
For summer themes, could do beach science? If you have a beach handy. Ooh or drought tolerance inducing endophytes, that's a bit planty/summery Does the fuzz/no fuzz gene affect size? Peaches seem to be bigger than nectarines in the shops here (although yours look similar sizes)
I wonder if this is really the only difference. I feel like even if this is the only genetic change nectarines are usually picked/sold smaller and firmer.
So, if pollen from a homozygous peach were to fertilize a nectarine flower, would the fruit be fuzzy or not? I'm thinking not. Nectarine trees produce nectarines.
Does grafting eventually change the DNA of a plant if you move down the generations? example, graft plant B onto plant A Take seed of grafted Plant B and Grow Plant C Graft plant C onto plant A take seed of grafted plant C and grow plant D etc, will at some point the DNA of the new graft take on some of the DNA of Plant A? pretty sure this is common with apples.
Great video! In 1997 I did a lab on the Lac operon gene on E. coli when I studied biochemistry. You mean that science hasn't come up with a better solution to electrophoresis and messing with that gel shit yet?
Can you explain how cultivars work? I just learnt that cabbage, kale, Brussels sprouts, broccoli and cauliflower are all the same plant (i.e. same species). They are all cultivars of the same plant. Is it similar to how we have numerous breeds of dogs, but they are all the same animal (i.e. same species) or cultivars are something different?
Great video ! It was so captivating and intersting(it is the first time I see a genetics experiment done in a lab), I couldn't stop smiling while watching it....genetics is simply incredible. I hope to be able to study this after I finish hight school. Can you make a video about the genetics behind the evolution of wheat ? If I remember right the names(and I write them correctly :)), it was from T. monococcum(diploid) to T. aestivum(hexaploid)
I would love to do some DNA insertions with you. It would be fun to see what little mutants we would have together. You look to have some real great DNA, I wonder if you are a peaches or nectarines.
I heard on a radio talk that there may be evidence that rice in Asia is descendent from ancient grains cultivated by Australian Aboriginal. Could you use DNA testing to investigate this? The radio talk was about the book Dark Emu, Black Seeds: Agriculture or Accident?
Is that single gene responsible for all the differences between peaches and nectarines? or just the fuzz. Like they taste different, they have different textures, different smells ... Fantastic video btw.
Oh no, there are additional genes that give both peaches and nectarines different characteristics. Both come in yellow/white varieties, some have different tastes, etc. But this is the gene that defines the "nectarine" phenotype.
You should see if you can do a video on DNA Barcoding! See if you can replicate the study that shows most supplements don't contain any of the item advertised on the bottle.
But if that's all it is how come the insides taste slightly different..? Peaches tend to be more juicy and softer, plus a slightly different overall taste inside
There are additional genes that can differ between varieties of peaches and nectarines that give different colors, textures, taste, etc, but this is the one that defines the "nectarine" phenotype.
So freaking cool! haha and what a lovely clear gel result to look at. I really appreciate it after looking at confusing diamers/monomers for weeks on gels
I love to see these byte-sized science videos back! How about some more story on genetically "evolved" / modified fruits in general? Like how science achieved the seedless bananas we eat nowadays (probably similar to your seedless watermelon video).
Does that mean a heterozygous tree could produce both peaches and nectarines depending on what pollinates it? Could it be growing both at the same time?
I looked through the paper and didn't see the length of the inserted segment. Do you know how many base pairs were added to make the recessive allele? Also, does anyone know where the additional DNA came from: bacteria, virus, transcription error, etc.?
It looks like it was from a retrotransposon! Transposons are chunks of DNA that hop around in the genome, and seem to be especially abundant in plants. The "retro" in this one means that it has an RNA intermediate when doing it's "hopping." I'll have to go back through the paper to figure out how long the insertion was.
Very nice and knowlable but it's little complicated...its would better if you explain it benefits for an ordinary person in simply language..but nice work
Oh yeah! thats SO cool, I mean, to SEE the science working, the results on the screen after all the process. Even though we know how it works and that it should produce some specific result I always get that excitement when i see the thing that I've put there to do something really doing that. I dont know, it's like when you have that idea in your mind but you really see how it looks like when you say it out loud or write it down. Keep up with that kind of videos. awesome!
But how could this genome insertion have happened many thousands of years ago? I mean, nowadays we've got labs for that kind of stuff and you could probably do it in your lab, but how did this happen in a world where humans hadn't even discovered technology (or only rudimentary ones like the fire or maybe the wheel)?
Because nature did it, not humans! This all happened within the peach. Small or large DNA changes like this are how organisms can acquire new traits and evolve!
The paper talks a bit about it coming from a retrotransposon (from another comment I made: Transposons are chunks of DNA that hop around in the genome, and seem to be especially abundant in plants. The "retro" in this one means that it has an RNA intermediate when doing it's "hopping.") Now if that sounds a lot like a virus to you, you're right. In the paper, they mention that they find many areas that "match" this insertion in other areas of the peach genome, "thus confirming the existence of other copies of this LTR-retroelement in the reference peach genome." It is unclear to me from what they've written if they believe that this is the first instance of that retroelement (which then "jumped" to other places in the genome) or if one of those other locations "jumped" to this fuzz-gene.
This was great, thanks, been searching for "peaches nutrition facts and health benefits" for a while now, and I think this has helped. You ever tried - Giyaarter Unflappable Gratification - (should be on google have a look ) ? It is a great one off guide for learning what to eat to balance hormones and eliminating trouble fat spots minus the headache. Ive heard some extraordinary things about it and my neighbour got amazing success with it.
Seedless grapes do have seeds but they have a mutation that stops a hard layer forming. They reproduce by humans making cuttings and grafting parts of these mutants onto a root system of a grape vine. In Europe most grapes are grafted onto american grape vine roots to stop an infection that is very virulent to european grapes.
KiloOscarZulu Bananas have very tiny seeds that you don't notice you're eating, right? Just like, strawberry seeds, they too are hardly noticed when eating the fruit.
Wow, awesome video! I'm so ignorant about genetics that a video like this, with your amazing ability to communicate well, made my day a bit brighter :) thank you!
I love this kind of stuff...I love knowing about the fruits that I love so much. You're very good at this...very smart and informative...and you're cute too!😇