OpenFlow: Multicolor Flow With Compensation Using a CytoFLEX LX

Подписаться 2,8 тыс.

Просмотров 5 тыс.

50% 1

In any multicolor experiment, we will need to account for spectral spillover using the process of fluorescence compensation. In cytometers
that allow users to change voltages or gains, adjusting the fluorescent detector settings is a critical step as this will affect the compensation
values. In this webinar we will use the CytoFLEX LX flow cytometer and Slingshot Bio's TruCytes™ TBNK Controls & SpectraComp® Compensation Beads to demonstrate the setting of gains and how to calculate compensation using the CytExpert software.

Наука

Опубликовано:

22 окт 2022

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 6

@yanxiachen8258 Год назад

Thank you so much for giving this lecture!! As a flow beginner, I found this is super helpful!!

@safaamashal7545 2 месяца назад

Hello everyone, I'm seeking advice on an issue with BD FACSLyric flow cytometer. I used the 7 beads and the 5 beads for creating lyse wash and lyse no wash settings. After configuring the settings, I conducted a compensation check using the same tubes as single-color controls and noticed problems with both overcompensation and undercompensation. I used the median values to check this. The CS&T results were good and Passed. Has anyone else experienced this? Any insights or recommendations would be greatly appreciated!".

@kathydaniels1077 2 месяца назад

Hi Safa! Thank you so much for reaching out. Unfortunately I personally don't have experience with the BD FACSLyric flow cytometer. When you ran the single color controls can I ask if you made sure you used the same antibody fluor conjugate as what was in your panel? Did you check to make sure that the single color was as bright as your experimental sample? The CS&T passing performance check is great, but I'm more curious about how the single colors were compared to your full stained sample.

@nouralzayed 10 месяцев назад

can use the kit on other tissues not just blood?

@DerekDaviesCytometry 8 месяцев назад

We used the Slingshot particles to demonstrate the principles of compensation. These principles will be the same for any other tissues but the particles themselves are suitable for blood markers only.