PCR Troubleshooting: Explanations and How to Fix Common PCR Problems 

I can't find a good way to thank you ☺️ you saved my life seriously I have to do a prasentation and really just know few about trouble shooting. Thaaaank you so much

I´m really thankful for this amazing video, it was very educative and helped me a lot to discuss the results of the PCR with my classmates. Good job

Good job. I've been in the business for over ten years and still learned a thing or two.

this was soooo helpful. thank you

Great video. I just have on comment on negative controls. What you've meant is a no-template control. A negative control contains DNA that is the "wrong" template for your primers, for example from a different species, that doesn't contain that target gene region.

This was very informative and I learned a lot.

Super quick and helpful! Thanks!

Good job madam ❤ thank you

Very informative and helpful video alot of thanks 🌹🌹🌹

That was very helpful, thank you!

Thank you for making this video, very helpful!!

This was really helpful, thanks a lot !

Very helpful thank you.

It's very well explained. ❤️

This was extremely helpful!

Thank you 🙏

thank you so much

awesome! thanks!

You're great teacher. But, my question is when problem no 1, solution is reduce primer. What do you mean that receipt mix or dilution primer that have to turn down?

Thank you mam.....❤❤❤❤

I have the protocols for normal PCR.how can I optimize them for using multiplex PCR

@2.50 what agarose gel electrophoresis conditions that you used? Thank you so much.

Please what do you mean by re-isolate template DNA?

Good

Trank you!!!!!

Good job madam ❤ thank you

Thank you so much