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Performing principal coordinate analysis (PCoA) in R and visualizing with ggplot2 (CC186) 

Riffomonas Project
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28 авг 2024

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Комментарии : 37   
@shillawolf4686
@shillawolf4686 Год назад
I am working on a thesis project, and i had to run pcoa. But i had no clue as to how and your video really helped me to understand what i should do and why these steps are taken. Know that your videos are helping a lot of students out there to better understand how to perform statistical analysis and we are very gratefull!
@andreamaritin2856
@andreamaritin2856 Год назад
Thank you very much! I was strugling with r projects in my master's degree and you just saved my grades.
@audreythomas4324
@audreythomas4324 9 месяцев назад
I wish I had found this before 2am 😅this explains so much better than anything else I've seen
@zmac18
@zmac18 2 года назад
Just in my time of need! Thank you!
@Riffomonas
@Riffomonas 2 года назад
Wonderful - I’m glad you found it helpful!
@cristianjaviermena6955
@cristianjaviermena6955 Год назад
awsome doc! thanks a lot. from argentina watching! and learning
@absartalat
@absartalat 2 года назад
Thank you for this. Looking forward to NMDS lecture.
@Riffomonas
@Riffomonas 2 года назад
Thanks for watching Absar!
@russtin1
@russtin1 2 года назад
My heart sank when you hard coded those numbers. I should have had more faith in you.
@Riffomonas
@Riffomonas 2 года назад
Ha!
@haraldurkarlsson1147
@haraldurkarlsson1147 Год назад
I agree with base R plots vs ggplot2 but still it is good to know. Many of the packages out there still use base R - especially the older ones. :(
@djangoworldwide7925
@djangoworldwide7925 Год назад
OMG i got sick when you hard coded the axes :OO thank god you fixed it :P
@kattiacristina
@kattiacristina 2 года назад
Great!! Thanks for this video
@Riffomonas
@Riffomonas 2 года назад
My pleasure! 🤓
@giovanniwidmer29
@giovanniwidmer29 2 года назад
Pat, at the risk of sounding professorial, it's principal, not principle. Thanks for the excellent education material!
@Riffomonas
@Riffomonas 2 года назад
Gee whiz. Thanks 🥴
@valentinberrios5927
@valentinberrios5927 Год назад
Hey thanks so much!, It captures my attention the fact that there is lower explained variation by the two first axis in comparison with PCA analysis. For my understanding , it is more acceptable when the sum of the explained variation by the two first axis is greater than 50% in PCA. It seems like it is a tendency to get lower explained variance in PCoA isn't it ?
@jiayunzou9867
@jiayunzou9867 2 года назад
Thanks for this wonderful video!! If we need to extract the first two axes for analysis of microbial community composition. I was wondering which one is better: PCoA or NMDS? Really looking forward to your reply, thanks a lot!
@Riffomonas
@Riffomonas 2 года назад
Probably PCoA
@jiayunzou9867
@jiayunzou9867 2 года назад
@@Riffomonas Thank you for the timely reply, it helps!
@sven9r
@sven9r 2 года назад
lovely! How do you feel about PCoA vs NMDS in metabarcoding studies? I think the question of which ordination is appropriate for the given data, is one of the most common source for mistakes in modern ecology.
@Riffomonas
@Riffomonas 2 года назад
I prefer nmds but I’m only using them to make pretty pictures
@sven9r
@sven9r 2 года назад
@@Riffomonas ty looking forward to the next episode :) have a nice weekend
@rojalinbiswal6723
@rojalinbiswal6723 2 года назад
when I am trying to plot getting error so how can I find your data ie., sample data to run the same code ? please answer .
@Riffomonas
@Riffomonas 2 года назад
You can find the link to GitHub at www.riffomonas.org/code_club/2022-02-10-pcoa
@alessandrorosati969
@alessandrorosati969 Год назад
How is it possible to generate outliers uniformly in the p-parallelotope defined by the coordinate-wise maxima and minima of the ‘regular’ observations in R?
@Riffomonas
@Riffomonas Год назад
Sorry but I'm not sure I understand what you're asking. It's likely beyond what I was trying to teach in this episode
@manueltiburtini6528
@manueltiburtini6528 2 года назад
Don't you need to perform Bartlett sphericity test or KMO test before? What's about triangle inequality and Calliez correction for negative eigenvalues?
@Riffomonas
@Riffomonas 2 года назад
When done with a distance like Bray-Curtis those are not performed
@priyankamishra3632
@priyankamishra3632 2 года назад
how to plot a pcoa for pathways instead of showing the samples?
@Riffomonas
@Riffomonas 2 года назад
Thanks for watching! You’d have to have a similar matrix but where the pathways are on the rows in place of the samples or in the columns in place of the taxa. What you do depends on what you want each point to represent
@priyankamishra3632
@priyankamishra3632 2 года назад
@@Riffomonas Thank you for the clarification. I'll try in this way.
@xiaojunslefteyebrow
@xiaojunslefteyebrow Год назад
8:56 how can I do this step to get positive eig values in Python? I did the rest of the project I’m working on in Python
@xiaojunslefteyebrow
@xiaojunslefteyebrow Год назад
please?
@Riffomonas
@Riffomonas 5 месяцев назад
I don't know python
@rajaldebnath6007
@rajaldebnath6007 2 года назад
Rival software package: QIIME. 😂
@Riffomonas
@Riffomonas 2 года назад
😉
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