Plasmid DNA Extraction (Traditional Alkaline Lysis Method) 

Super helpful, you're supposed to do all these steps to separate the DNA from certain bacterial contents like proteins and lipids, all these make a lot more sense now, at the end we precipate the DNA with the alcohols and then dilute them for further analisis in the TE solution. Thank you very much.

Thanks.This video is very informative and every steps is shown easily , we want to know procedure of plasmid curing.

I got to the isopropanol step and centrifuged it, when I took it out of the centrifuge I couldn't see a pellet. I think I might've left it too long. So if I centrifuge again could i potentially Iget a pellet?

Thanks a lot I really find this helpful

Please also indicate the temperature of the centrifuge

What is TB?

Thanks it is useful 😀

what are the observations after adding solution 2?

good one

خب بابا میدونیم میدونید بیدارم زنگ زدن نمیخواهد که

worst protocol ever,worst time management..i isolate around 10 plasmids in this mch time and if you use RNAse at the start you must use a co-precicitant...shit protocol..