Protein Purification 

Protein Purification aims to isolate a single type of protein from a biological tissue or culture. This video explains the most common methods applied to purify proteins, which are:
Precipitation methods, filtration methods, electrophoresis and chromatographic methods.
It is important to have an overview of these techniques in order to choose which method/methods suit the project best. Factors that can influence such a decision are: the desired level of purity, the accessibility of required equipment and the characteristics of the target protein. In practice multiple purification techniques are often combined.
Time Code:
00:00 - Intro
00:20 - Content
00:49 - What is protein purification?
02:20 - Separate your protein from a biological tissue
02:53 - Choosing the right purification techniques
03:40 - Precipitation methods
03:58 - Salting out
05:10 - Chromatography
06:08 - Affinity chromatography
07:25 - Ion Exchange Chromatography
08:07 - Hydrophobic Interaction Chromatography
09:08 - Size Exclusion Chromatography
10:20 - Filtration Methods (Dialysis)
11:12 - Electrophoresis
12:08 - Native PAGE
13:29 - Outro
At Cube Biotech we are experts in the purification of protein with over 40 years of experience. We offer a wide range of protein purification products:
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Furthermore, we offer custom protein purification:
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For general inquiries about our company check out:
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For more detailed information about protein purification techniques check out:
Liu, S., Li, Z., Yu, B., Wang, S., Shen, Y., & Cong, H. (2020). Recent advances on protein separation and purification methods. Advances in colloid and interface science, 284, 102254. doi.org/10.1016/j.cis.2020.10...

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