Hi. By using the image lab software, can you select and work with 2 or more bands per lane. For exemple if my antibody recognizes 3 isoforms of a protein and detects 3 bands. ? Thanks
I've been playing with this and I'm almost certain that most people don't run a serial dilution of a given sample to validate quantity vs signal or band intensity (area under the curves) vs amount loaded. ie. What does a 20% increase or decrease or increase look like? I played with the exposure/maximum signal before saturation, and I get the best linearity of the antibody signal/quantity when the exposure is only 20-30% of the maximum.
Honestly this is good practice, but undoable in today's conditions. A regular lab doesn't have neither the time nor the ability to keep up with all the controls. I blame the mindset of fast publishing that pushes you to do quantity over quality.
