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Refolding of Inclusion Body Proteins from E Coli 

Creative BioMart
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This video explains how protein fold in vivo and inclusion bodies refold procedure. It also introduces IBs refolding methods, such as dilution and chromatography.

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1 окт 2024

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Комментарии : 10   
@sabrinarun2515
@sabrinarun2515 7 лет назад
Fantastic video about the Refolding of Inclusion Body Proteins from E Coli, which is the main subject of our research. We also want to learn more the detailed information about its processes.
@andreabrook7581
@andreabrook7581 7 лет назад
However, now the refolding of inclusion bodies has become a bottleneck in biopharmaceutical.
@candyswift9088
@candyswift9088 7 лет назад
What refolding method do you suggest for cost effective purpose?
@MrCreativebiomart
@MrCreativebiomart 7 лет назад
Dilution is the cheaper way, but the effect is not as good as chromatography. And the subsequent concentration is also laborious.
@stevenrios6236
@stevenrios6236 7 лет назад
Is it necessary to establish efficient refolding protocols?
@MrCreativebiomart
@MrCreativebiomart 7 лет назад
The protocols for dilution or chromatography methods are commonly used. The most important is the buffer and other conditions you used in the process.
@carolinegreen4771
@carolinegreen4771 7 лет назад
Do you have general protocols for protein refolding?
@MrCreativebiomart
@MrCreativebiomart 7 лет назад
Unfortunately, no. The process is similar for different proteins, however, the condition details various greatly.
@adarshguptak
@adarshguptak 3 года назад
Auto voice is creepy, as if she's going to refold me.. left after a minute
@okpolihenry983
@okpolihenry983 2 года назад
true
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