for the past 4 years, your channel have helped me through my hard times until i finally got my degree. Now look, this is my first time working and i'm still here. i still need you. THANK YOU MISTER!!! i hope you have a happy life! now and always!
This was a topic that could have been confusing and hard to understand but you managed to explain it clearly and it was easy to follow your explanation! Great Job!
I probably never get the chance to tell you in person that YOUR lectures are the main reason of my GPA =8 in my mater degree...YOU are just awesome man......
about F=Fv：Because friction is more complicated, it is usually some empirical formula. In the past, the friction force you were in contact with usually referred to dry friction between solids. If you consider that the object is moving in air or liquid, the measurement will find that the friction force is related to the speed. It is generally considered that at high speed, the friction force is proportional to the square of the speed, and when moving slowly, the friction force is approximately linear with the speed.
Not be a nerd, but SDS is missing one more carbon in the video (Dodecyl = 12). I only see 11 carbons in the drawing. I appreciate your work, and needed this very much.
Thanks for a great video. I have one question. How is the force of friction = f*velocity? Isn't frictional coefficient dimensionless so the unit does not come out to be Newtons?
It occurs to me that the structure formula of SDS written on the white board is incorrect (one "CH2" is missing in the dodecyl fatty acid)... But otherwise: great explaination!
I think there is a problem, the coefficient of friction must be without dimension, while here you when you multiply f with velocity it does not make sense, would please clarify it???!!!!!
He is right about the electric field being constant, BUT! Actually the voltage is not constant! In order to maintain a constant electric current, which generates ''almost'' constant velocity, the gel-electrophoresis machine increases the voltage during the process in order to resist the ever increasing impedance values inside the medium (anyone doing gel-electrophoresis can see that). Actually during the migration the voltage more than doubles! Does not matter in the end... Excellent video anyway!
I always thought the reason ads page was due to size not charge was because the charge to mass ratio for all proteins is the same with SDS. 10 amino acids = every other amino acid gets sds/ = arbitrary net charge of 5. Divide that by 10 = 1/2. 30 amino acid... 15 charge.. 15/30 is 1/2 .Thus all proteins have the same exact charge/mass ratio.
amazinnnnng. can i ask a tow question: 1. whats the deference between 6x and 2x loading dye and which i should use ? 2. which voltage is optimal to run one caste of gel, i usually use 65 v. ?
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
the concentrations of chemicals in the 6x loading buffer is three times as high compared tot the 2x loading buffer. This is the only difference. You should dilute your protein sample with the loading buffer in such a way that you always end up with 1x loading buffer. the 2x loading buffer should be diluted 1:1 with your sample. the 6x should be diluted 1:5.
Farida Chy the presence of mercaptoethanol disrupts the disulfide bridge bond found in the protein when mercaptoethanol is not present the disulfide bond in protiens stay intact hope that was helpful
Mercaptoethanol is a reducing agent that cuts disulfide bonds within proteins in order to disrupt the 3-dimensional shape of proteins, a process that is called "denaturation".
Can anyone please explain Why frictional force is => frictional constant(f) X velocity (v)? Far as i know Frictional force is equal to frictional constant(f) X Normal Force (N)
