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Single-cell sequencing explained in 2 minutes 

Sanbomics
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What is single-cell sequencing? Why do single-cell sequencing? Single-cell sequencing is a complex process, but the fundamentals are relatively straight forward. Here I cover the basics which are needed before getting your feet wet in more complex single-cell topics.
Single-cells sequencing can be broken down into just a few basic steps:
1) Label an individual cell's RNA with unique barcodes
2) sequence the RNA from all cells and split the reads based on their attached barcode which are also sequenced
3) Downstream analysis...

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19 июн 2024

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Комментарии : 23   
@cold_hardfacts
@cold_hardfacts Месяц назад
Im not a scientist. I came here from cancer research. I gave it a thumbs up. Clear and informational.
@roshanpatel06
@roshanpatel06 10 месяцев назад
Your videos are perfect. I appreciate the no-intro, straight to the meat style.
@keeganhart2308
@keeganhart2308 Год назад
Great brief description. Thanks!
@sanbomics
@sanbomics Год назад
You're welcome!
@michaelbailey9643
@michaelbailey9643 Год назад
Great video and summation. Surprised it doesn't have more views!
@sanbomics
@sanbomics Год назад
Glad you liked it!
@szymonjakubowski3574
@szymonjakubowski3574 Год назад
Awesome! Very clear explanation, thank you
@sanbomics
@sanbomics Год назад
Thank you!
@sagek7949
@sagek7949 Год назад
This was helpful. Thanks
@sanbomics
@sanbomics Год назад
Glad it helped!
@jappe008
@jappe008 Год назад
Thanks for the video! Could you maybe elaborate on common use cases for single cell sequencing? E.g. what questions could you answer after analysis? Just curious of the bigger picture where scs fits Thanks!
@sanbomics
@sanbomics Год назад
Great question! There are many potential use cases, but they all more or less boil down to one core concept: each tissue you type you examine has multiple cell types which all behave or react in their own distinct way given a given context or stimulus. Here is a quick examples, but there are many more you can extrapolate this key idea to: Is there a specific lung cell population that responds uniquely to infection?
@kamarsedik1448
@kamarsedik1448 9 месяцев назад
Could you suggest me a simple reference for beginners regarding scRNA_Seq
@sinamt2982
@sinamt2982 11 месяцев назад
Thank you so much!!
@sanbomics
@sanbomics 11 месяцев назад
Glad it helped!
@neurostudywithme
@neurostudywithme Год назад
Is UMI shows nFeature or nCount in the sc rna analysis?
@sanbomics
@sanbomics Год назад
typically nFeatures are the number of genes and the nCount are the number of UMI
@neurostudywithme
@neurostudywithme Год назад
@@sanbomics thank you so much!!
@saveriov.p.7725
@saveriov.p.7725 Год назад
i dont get how the bead somehow tags each RNA transcript in the cell if it just sticks to the outside?
@sanbomics
@sanbomics Год назад
The bead, a cell, and the reagents all go in one droplet. Then the cell gets lysed and they are all swimming together
@ShenStefan
@ShenStefan 8 месяцев назад
@@sanbomics Does each bead contain many DNA segments with the same barcode and different UMIs?
@user-fo8we8ur4n
@user-fo8we8ur4n Год назад
Why is it easier to understand on what you are saying than to understand it on my language hahaha,,
@sanbomics
@sanbomics Год назад
Hahah xD People just like to make it more complicated than it really is!
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