Single Molecule Sequencing: Dynamics of M6A in Viruses 

Instructors: Stacy Horner, Associate Professor
Department of Integrative Immunobiology Duke University School of Medicine
Lecture Summary: Are there epitranscriptomic modifications on viruses? In this lecture, Dr. Horner summarizes the importance of RNA modifications in basic scientific research, introducing m6a as the most common mRNA modification, along with related methodologies for capturing m6a in high-throughput fashion. She then summarizes her own lab's work in elucidating the role of m6a in viruses, reviewing current challenges to the field.
Course Summary: Sequencing-based research has become the dominant investigative practice within the biological sciences. Single-molecule sequencing: training, methods, and applications, responds to this development by providing a hands-on and in-depth introduction to sequencing for students, from Sanger to third-generation technologies. Utilizing an alternating lecture-lab schedule, students are introduced to fundamental basic principles of DNA & RNA science, progressing to cutting-edge library preparation and sequencing analysis techniques with Illumina and Nanopore technologies. Students will have the opportunity to perform direct-RNA sequencing samples on research samples, and experience first-hand the ethical implications of this data. Relevant concepts in biology and computer science will be addressed.
GitHub Link: github.com/The...
Relevant Papers:
Sacco MT, Bland KM, Horner SM, 2022. WTAP Targets the METTL3 m6A-Methyltransferase Complex to Cytoplasmic Hepatitis C Virus RNA to Regulate Infection. J Virol 96:e00997-22.
doi.org/10.112...
Aufgebauer CJ, Bland KM, Horner SM. Modifying the antiviral innate immune response by selective writing, erasing, and reading of m6A on viral and cellular RNA. Cell Chem Biol. 2024 Jan 18;31(1):100-109. doi: doi.org/10.101... . Epub 2024 Jan 3. PMID: 38176419; PMCID: PMC10872403.
Horner, S.M.; Thompson, M.G. Challenges to Mapping and Defining m 6 A Function in Viral RNA. RNA 2024, 30, 482-490, doi.org/10.126...
Course website: physiology.med...
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