I have to watch your lectures before I try and understand any concept my lecturer is trying to explain because I know you're going to explain it in a way I can understand, you are an incredible teacher!
just for information aminoacids for synthesis, are purchased with t-boc or Fmoc protecting group already attached to backbone amine. Also side chains have protecting groups. DCC is a good activator for backbone carboxylic acid, there are also other activators that could be used.
Btw on that top DCC molecule your nitrogen has 2 lone pairs, 2 sigma bonds, and 1 pi bond - though it should have one less lone pair as a group 5 element if it's neutral as you've indicated
Brilliant video! What is it about TFA that makes it so effective at removing t-butyl derived protecting groups? Also, why does the HF added at the end target the phenyl specifically at the C-terminus, and not the other phenyl moieties in amino acids such as phenylalanine? Thanks!
Hii there, nice video. I am bit confused on my synthesis as I got a sequence using phage display library against a pathogen for example: STFSQNG (N to C) and I want to synthesise it using Fmoc SPPS. But as the FMOC SPPS using Rink amide resins generate from C to N, should I use Glycine as first amino acid (reverse) or use S (serine) as first amino acid and then T,F,S etc.
I think it's important to learn the mechanisms of these reactions instead of just the products. My professor makes us memorize the reaction mechanism for protection with F-moc, DCC, etc. Even the mechanism of the deprotection of F-moc with treatment of mild base.
Simpel and straightforward explanation. However, using "actual" ever 5-10 s is a little annoying. If you could reduced it and only use it, when you want to underline something it would improve your point :) All in all, good work bud! Keep it up.
