In this second video of the series dedicated to somatic embryogenesis, we show how is the process of obtaining the explants used in our experimental system: complete stamens of grapevine.
The formation of somatic embryos through in vitro culture is probably one of the most relevant biotechnological tools in current plant breeding. The process of development and establishment of an efficient somatic embryogenesis protocol is not the same in all species and, in fact, there are no protocols for all of them (not even far). However, there is a set of steps that are common to most procedures. In this series we will show how are the key methods that we use in our laboratory, all of which are outlined in the following 4 videos:
1.Preparation and sterilization of plant material
2.Extraction of the explants used for the induction of the embryogenic response
3.Sowing of complete grapevine stamens in induction medium
4.Multiplication and differentiation of somatic embryos
-------------------------------------------------------------------------------------
To go into more detail (culture media, times, results, etc.), the references of the articles on which the whole process is based:
- Acanda Y, Prado MJ, González MV, Rey M (2013) Somatic embryogenesis from stamen filaments in grapevine (Vitis vinifera L . cv . Mencía): changes in ploidy level and nuclear DNA content. In Vitro Cell Dev Biol Plant 49: 276-284.
- Prado MJ, Grueiro MP, González MV, Testillano PS, Domínguez C, López M, Rey M (2010) Efficient plant regeneration through somatic embryogenesis from anthers and ovaries of six autochthonous grapevine cultivars from Galicia (Spain). Sci Hortic Amsterdam 125: 342-352.
-------------------------------------------------------------------------------------
Researchgate: www.researchga...
Website of the research group:
mrey.webs.uvigo...
16 сен 2024
