Southern blot | Biomolecules | MCAT | Khan Academy

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Опубликовано:

16 июн 2024

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Комментарии : 75

@mrsandman939 8 лет назад

Yes but you missed out one important part. NaOH is added to those double stranded DNA fragments to denature them, otherwise complementary probe wont anneal to the target sequence

@mcdonads11 7 лет назад

saxeli gvari thank you!! i was a bit confused but thanks for clearing it up :)

@abidabdelaziz9936 7 лет назад

exactly what i was thinking :D !

@mezzomaxZ 6 лет назад

@jessicayakobashvili6536 6 лет назад

madlobt :)

@michaelbrandt5155 5 лет назад

Yo thank you

@NisthaAtishLimitLesss 6 лет назад

Simplified and the best video lecture on southern blotting .

@amineabdz 2 года назад

You forgot the most important step, which is denaturing DNA ( from double strand to single strand ) in order to allow Hybridation between the DNA fragments and our DNA probe... which is followed by a rigorous washing to remove all excess probes that aren't attached.

@ariansajjady1785 5 лет назад

What a short great video!My biotech professor confused the f out of everyone! She also took 20 minutes doing that!

@Elixir.dm. 5 лет назад

Best video on u tube for southern blotting...thanks a lot

@mayko2468 5 лет назад

Explaining the process step by step definitely helped a lot (sth many teachers can't do in 2 hours) but Plz update this video by adding the denaturation and renaturation step and other important details

@mariapaulapedromorgado774 7 лет назад

Amazing! Thank you so much

@xMaria72x 8 лет назад

Really helpful video. Thanks!

@tuanngocnguyen2878 6 лет назад

great explanation. love your channel.

@sumaiyaislam1152 6 лет назад

veey well explained ..thanku sir

@johannesmthembu8663 6 лет назад

Thank you well explained!!!

@user-vj2et1ch3r 5 лет назад

It was very helpful though kinda sleepy without any accent to some important points... And yes for the best reply. NaOH or any other alkali treatment would make Dna denature.

@BlackLukeS 8 лет назад

Very very helpful. Thank you

@tantralin735 8 лет назад

tks~~helpful~~

@isidoratasic2058 5 лет назад

Excellent!

@rimayousuf6372 8 лет назад

thank youuuuu

@RemiTa1st 8 лет назад

I wanna cry :') that was very helpful thanks I love you

@maralingamaralinga 2 года назад

You forgot to mention one important step. After annealing and before exposure to UV, you need to wash away excess radioactive labelled DNA

@adhithyapoyyamozhi4801 3 месяца назад

You are awesome!!!

@mohitgangrade5491 7 лет назад

nice one @khanacademy. but u missed the NaOH step which is important for denaturing. besides that it was great

@dihu5635 6 лет назад

better than my professor's PPT

@oumaima353 3 года назад

Thanks a lot

@haluk2842 Год назад

so filter is STURDIER than the gel?

@mohdmushfiquekhan5003 3 года назад

Good information

@uzithobilesifuba3105 8 лет назад

Thanks , this is so helpful

@nanone1994 9 лет назад

very well explained :) thx buddy

@prateekmisra8251 3 года назад

Sooooo......just curious, where are you now?

@sjian1026 2 года назад

@@prateekmisra8251 o.o

@sciencenerd7639 2 года назад

thank you

@khalida02 6 лет назад

he didn't mention that #3 is referred to as the hybridization step

@peggycarter6460 4 года назад

but what if the gene A was let's just say : CATCCGTA so the probe would be : GTAGGCAT and another gene fragment had a common part like this : CATCCGTAGGT so the first bases are the same is there a chance that the probe would anneal to another gene other than gene A? or even to one where it has a common part of gene A but a shorter fragment ? even if the difference was one extra base ? or the electrophoresis eliminates that possibility? help, please .

@Banamol 2 года назад

If we are studying a certain protein, there are going to be few genes that will fit in exactly with the probe for our DNA of interest. That being said, most prob lengths are more than 100 bases long. That makes it difficult for things to overlap.

@uni_versetones 5 лет назад

Missed blocking step before labeling with probe.

@maryamrahim342 2 года назад

To the piont .

@MrTRANnysaurus 9 лет назад

how does the DNA probe anneal to the the fragments? after restriction enzymes cut are they single stranded?

@xGodzAssassin 9 лет назад

Yes the gel is treated with alkali that separates the 2 DNA strands into single strands

@hoanguyen-wk4je 6 лет назад

I couldnt agree with you more, this is a hibridization in which single stranded cDNA/ RNA will bind to another DNA/ RNA (the one we want to detect) in order to form a hibrid

@ArunaBoroMimi412 6 лет назад

the probe used is complementary to the targetted gene

@96wanny 8 лет назад

what is the aspect difference between the three method ? northern, southern and western . i only find a few differences bethween them ? Can you identify the difference>

@Lia-in1ts 7 лет назад

basically , Southern is used to detect DNA ,Northern is for RNA and Western for polypeptides.

@user-vu8hb7zv1j 7 лет назад

what if it happens that the gene is cut into different fragments which are located in different parts on the gel? Will radiation be detected in several spots then or none of the spots?

@muhammadadnankhan8602 3 года назад

None of the spots...because our probe can anneal to a complete gene having it's complementary bases,not fragments of it

@alexyoalexsup 7 лет назад

genie.

@naotomori7598 8 лет назад

are piece of DNA=DNA fragments also ds? if yes, how radio-labeled DNA anneals o.O?

@Ventsi24 8 лет назад

+Nao Tomori It has complimentary bases to the DNA fragment so forms hydrogen bonds with it

@rafajuerto1009 8 лет назад

+Nao Tomori You denature the dna in the gel by soaking the entire gel in NaOH before transferring it to the membrane which makes the DNA single stranded. The probe is also single stranded.

@Batoul-obaid 5 лет назад

I can’t understand why we have to separate the dna molecules by gel electrophoresis?.can’t we just add the radio-labeled DNA and then expose to X-ray?

@jmca_power 3 года назад

"Joe electro phoe reese´s" now you can´t unhear it, you are welcome

@shreyaskulkarni5312 Год назад

🔥

@sccm100 5 лет назад

The volume is so low

@MultiSciGeek 2 года назад

I don't understand this. Where's the more in-depth explanation?

@sameervyas8375 4 года назад

How old are you when you are taught this in US?

@steveguzman375 4 года назад

Evil Assassin 3 months old

@ashatnawi91 5 лет назад

I'd appreciate this video more if I could hear what you're saying!

@wayne9267 8 лет назад

what if the enzymes cut the gene a in half so the complementary dna can not completely allign?

@rafajuerto1009 8 лет назад

+Wayne The enzymes will cut at a specific sequence of nucleotides every time. You will select the enzymes you want to use based on the sequence of your gene and you can ensure that the enzymes will cut in the correct place. It is possible that the enzymes will make a mistake but this won't be very often so the majority of the product will be of the correct length.

@scientificartchannel3146 4 года назад

The enzyme which is used here is called restriction endonuclease. The main characteristic feature of this particular enzyme is that after searching for a palendromic sequence of bases it will cut at specific locations just away from the centre of the palindromic sequence. It cleave the DNA into fragments and hence it called molecular scissors. Now coming to your question if the enzyme cut the DNA half then there will be error occurred hence it will soon proofreaded by other enzyme such as epsailon0 subunit.

@Swordsman0 7 лет назад

No. I'm Good

@mohammado.eshkalak3140 7 лет назад

These guy is wrong all over. He has just learnt about Southern Blot probably. Khan academy must Rethink on those selected for teaching these. I believe they are geetting paid good money so please present high quality stuff.