Sterile Technique 

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

Cooling sterile forceps with the plastic of a petri dish... what? You're going to have people melting plastic onto hot metal instruments...

Very useful video. Thanks for sharing!

Thank you very much

good vid, thank you.

thank You

Thanks!

Working in Addgene is my dream.

Wonderful illustrations of aseptic techniques used in the lab. Keep uploading the good stuff.

love her gloves

would a camp stove such as the msr pocket rocket be able to achieve the same effect as a bunsen burner for upward aerial bacterial draft / cone of protection?

1. Putting the plastic seals on the bottles in the flame as pictured will degrade them, and isn't at all necessary--the purpose of the flame isn't to burn or sterilize the bottle top, but to create an upwards air current near the open mouth of the bottle, preventing airborne contaminants from entering the bottle. 2. alcohol burns almost without heat so the metal doesn't get hot --the flame is used to dry the metal sterilized with ethanol, not heat it. Some people mistakenly heat the metal by keeping it directly in the flame of the burner (ie like when you heat a metal inoculation loop until red hot), and this will result in a burning hot metal utensil, but this is incorrect technique.

What a stunning actress! And such good technique!

Very helpful, thank you! I have never seen a candle jar used instead of a bunsen burner. Considering it for my lab. Why did you choose it? Where can I get one? Thanks!

Hi Bi308 students😁

𝚂𝚑𝚎 𝚜𝚑𝚘𝚞𝚕𝚍 𝚋𝚎 𝚞𝚜𝚒𝚗𝚐 𝚊 𝚐𝚕𝚊𝚜𝚜𝚎𝚜 𝚕𝚊𝚋 🙂

Cheer~~~not able to produce children or young.???😢

heloo madam

Sorry but I can’t take someone who clearly has no clue about aseptic techniques seriously when they’re trying to show others how to be aseptic in the lab/cleanroom lmao

Thank you very much