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STR (Short Tandem Repeat) Analysis and DNA Fingerprinting Example | Genetics

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In this video I go over the background of STR analysis and how it is used for things like crime scene analysis and maternal/paternal tests. I give a quick background, go over PCR (polymerase chain reaction), gel electrophoresis, and then check out two examples.
If you have any questions, let me know in the comments. Thanks and hope you enjoy!
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Опубликовано:

25 авг 2024

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Комментарии : 43

@kuotesforu 9 месяцев назад

18 minutes of Pure and easy to understand Knowledge 🎉❤

@kmacdowe 3 года назад

Thanks! Clear, concise, easy to understand description of a complex subject.

@subhashjuneja1689 2 года назад

That was very clearly demonstrated in a very simple language. Thanks

@meroproductions Год назад

Thank you for helping me pass t forensics test

@areebahassan6540 3 года назад

THANK YOU SIR. THIS WAS REALLY HELPFUL.

@shimaagaber8641 3 дня назад

جزاكم الله خيرا

@rabiaselli2073 3 года назад

Thanks.Really good explanation.

@DrWD40 3 года назад

You're welcome! Glad you enjoyed it

@yogeshkmrful 3 года назад

Very nice video! helped me in my presentation

@chubiin20s36 Год назад

EXCELLENT

@annelisse16 2 года назад

This was amazingly helpful

@dheerutiwari2771 Год назад

Awesome video

@eatonashton4962 3 года назад

very clear and helpful. Thx! :)

@knockoutthehurdles5305 3 года назад

Can we detect individuality of same male through Mt dna str analysis

@kaorbo 11 месяцев назад

How likely is it that a father and sons would show as a match to a dna sample ran through str pcr by sled?

@DrWD40 11 месяцев назад

The mother would also have to match at least the half the father gave.

@arjan8005 3 года назад

Awesome video. I was just wondering how would you design a primer so that it would bind to the start of the STR and not in the middle or near the end since the STR is just repeated base pairs. Thanks :).

@DrWD40 3 года назад

Before and after the repeat sequences there are just normal random bases. Part of the reasoning behind using the STRs we choose is the primer selection. Forward and reverse primers need to be designed to have similar annealing temperatures.

@arjan8005 3 года назад

@@DrWD40 Okay thats great, cheers :).

@pbraise1887 3 года назад

Hi ! What’s the interest to create billions dna fragment with PCR if we just use one of them for paternity test ? And I don’t understand why is it necessary to use gel electrophoresis ? Aren’t all the fragments the same ? Thank you

@DrWD40 3 года назад

You need lots of fragments for them to appear on the gel when you stain them. The more DNA you have, the better the resolution on the gel. Fragments should be different sizes between individuals because the amount of repeats vary from person to person.

@cynthiarodriguez7395 3 года назад

Can we use 2 or 3 different set of primers to detect different STRs in the same reaction so we can have more bands in the same well of the gel? I would like to try making one of those personalized dna profiling portraits/canvas they sell online, like in dna11.com . Is this the correct way of doing it or what would you suggest? Thanks !

@DrWD40 3 года назад

Possibly. Each primer has a slightly different annealing temperature so you'd want to design them to be as close as possible. When I did genotyping on transgenic mice I had to run my PCRs separately because the annealing temps were too different.

@TheCortymast Год назад

I just don't understand how exactly we only multiply the STR when performing PCR. Shouldn't the Taq polimerase just keep on running and duplicate the whole genome?

@DrWD40 Год назад

The forward and reverse primers determine the ends since that's what Taq starts. It requires that primer and won't elongate any open segment of a strand.

@danielafrausto9354 2 года назад

When does the Taq polymerase know when to stop? Like how do you isolate just the STR from the rest of the bp?

@DrWD40 2 года назад

You have a forward primer and a reverse primer that flank the STR. After a few cycles you'll only have the STR region with the flanking forward and reverse primary.

@europhile2658 3 года назад

good video but you have not explained the relationship between Alleles and STRs. Is an STR an Allele? or does an Allele contain multiple (or one) STR? thanks

@DrWD40 3 года назад

Yup! An STR is an allele. The number of repeats within the STR defines the allele.

@drsanchitadey-rh1zt Год назад

@@DrWD40 I a

@marcosgrove6296 3 года назад

Pls, answer my question. So can we conclude that STR use multiple-locus marker? coz the markers represent various loci along the genome. or STR using single-locus marker? bcoz at the first analyze, we only investigate single-locus that similar to our suspect, every variation that we generated is represented different allele in the investigated locus. Please answer this I got so confused STR belong to single-locus or multiple-locus?

@DrWD40 3 года назад

There are 13 CODIS core loci that they can choose from. Here they are with some more info www.barnardhealth.us/forensic-science/the-13-codis-str-loci.html