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The Home Scientist 024 - Making thin-layer chromatography (TLC) plates 

TheHomeScientist
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How to make thin-layer chromatography plates (TLC plates) for a few cents each that are as good as commercial TLC plates that sell for a dollar or two each. You can use these home-made TLC plates the same way you'd use chromatography paper, but the plates provide sharper separations and require far less analyte. They also lie flat, and are much easier to store for later reference.

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22 май 2010

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Комментарии : 31   
@joybaker5621
@joybaker5621 7 лет назад
Thanks! Great video. So helpful for teachers without much budget!!!
@mrericsully
@mrericsully 14 лет назад
I'm working on my supply orders for school next year and you just saved me a lot of money. Thanks for the great information.
@BrotherBloat
@BrotherBloat 14 лет назад
rich content as always - thank you!
@redband788
@redband788 8 лет назад
Excellent video.
@Fordi
@Fordi 14 лет назад
Pretty awesome info for the mad scientist on a budget ^_^
@miraclo3
@miraclo3 14 лет назад
nice video!
@earlross8537
@earlross8537 3 года назад
you are a legend, ty!
@olympicfan2
@olympicfan2 14 лет назад
very good!
@KristoffDoe
@KristoffDoe 13 лет назад
It's worth adding that previously reagents like Dragendorff or ninhydrin mentioned in your previous videos work beautifuly with TLC, giving an extra dimension for identification of substances. And even without reagents you can make nice separations eg. dyes and inks (which is used in forensic investigations of documents when there's a suspicion of forgery).
@zcuttlefish
@zcuttlefish 14 лет назад
Hey RBT thanks for putting this video up! I use this everyday in lab (along with flash chromatography). I had been wondering what the binding agent was (I would think that the solvents would dissolve the binder). I think it would be cool if you talked a little about the principle of TLC bc most people won't have the solvents, uv lamp, or stains necessary. Cheers!
@HERBIVOR1212
@HERBIVOR1212 14 лет назад
pretty interesting
@searodrig
@searodrig 10 лет назад
do you have a website where these instructions are listed? That would be awesome!
@skagsux
@skagsux 11 лет назад
Is it possible to use powdered granules from silica gel packs that come in a vitamin bottle or with your new pair of shoes (you know these desiccant packs). Also is it possible to use the immobilized phase you detail with highly hydrophobic (oily, resinous etc) compounds.
@DutchPhlogiston
@DutchPhlogiston 11 лет назад
Interesting. I wander how well your alumina/plaster mix would perform in a homemade HPLC-like column...
@0Krystal0Teque0
@0Krystal0Teque0 11 лет назад
what would u use for colouring as most tlc use fluorescent??
@petevenuti7355
@petevenuti7355 11 месяцев назад
I want to make TiO2 plates for experimenting with organic solar, i assume the process is mostly the same. Would you recommend the same binder? What else? What about sodium silicate?
@wubbalubbadubdub_
@wubbalubbadubdub_ Год назад
He mentioned that if we don't have access to alumina we can also use cornstarch or talcum powder. So can I use just about any type/brand of cornstarch or talcum powder or do I have to look for something specific? And can I just use it directly or is there some sort of process before? Hoping for a clear answer (I need to do this for a school project)
@spencerburrows3525
@spencerburrows3525 10 лет назад
what are the amounts again?
@annakowalska725
@annakowalska725 2 года назад
clever
@tyt0uoff146
@tyt0uoff146 2 года назад
Can i use calcined alumina or fumed silica ?
@sorathelight223
@sorathelight223 14 лет назад
sweet
@aoihana1042
@aoihana1042 9 лет назад
good blese
@vevenaneathna
@vevenaneathna 14 лет назад
kewl
@tim0090
@tim0090 12 лет назад
1.25ml per gram alumina and 15% of binder ???????????? so 1.25 grams of water to 1 gram of alumina to 0.3375 grams of binder or 1.25 grams of water to 1 gram of alumina to 0.15 grams of binder
@pauldell6984
@pauldell6984 8 месяцев назад
did you ever solve the mystery?
@temporarilyjosh
@temporarilyjosh 12 лет назад
How much does the binder affect retention? Is it possible to use this technique, using the same binder, with polymer-based reversed-phase media? Thanks for the great demo!
@XN-zy4xe
@XN-zy4xe 7 лет назад
if so made plate will glow in UV ?
@TheWanderingChemist
@TheWanderingChemist 6 лет назад
A slide prepared this way will not fluoresce or glow unter UV irradiation by itself (unless your substance is a fluorophore itself), for that you will need a Mn-doped Zn2SiO4 layer which will absorb UV light at 254 nm www.sigmaaldrich.com/catalog/product/sial/02554?lang=de&region=DE
@petevenuti7355
@petevenuti7355 11 месяцев назад
No, it's what absorbs into it that does or does not glow. Take several different color highlighter markers and mix up the colors in a small well (a slot as long as the width of the slide that's about 2-5 mm deep or so) dip the edge in , and the different colors will separate into bands and glow under UV as they climb up the slide before they dry.
@nehorlavazapalka
@nehorlavazapalka 14 лет назад
Fail, I've accidentaly arrived at this video while having the TLC plate in front of me.
@Bluetorchproductions
@Bluetorchproductions 14 лет назад
looks good, hey if it costs less to make them, make them yourself and sell them online for just under the store price and make a small buck =]
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