Kesel bgt denger presentasinya wkwk, kebanyakan aa ee emm presentasinya jadi jelek dan gak jelas wkwk. Malah lebih enak denger dosen Indonesia yg ngejelasin wkwk lol. Latih lagi public speakingnya ya mrs.
Great explanation. One minor very mistake is that samples 14 and 15 are not in duplicate wells. If they were, you would only be able to run 39 samples in duplicate rather than 40.
Crystal clear presentation! Thank you. Just one comment, in the last part, should the standards using antigens also (instead of any proteins), to quantify the 40 samples? In addtion, is it absorbance or fluorescence?
If you're using spectrophotometer it measures absorbance, which is what I believe they explained in this video. There are elisa methods where a flurophore is used, where fluorescence would be measured. Probably 2 years too late to answer your question though.
Hello!! Your video helped improve my understanding a lot :) I was wondering if you'd be willing to share your references for this topic (specifically the ones used for this presentation)? I'd like to read more about it. Thank you so much!
Thank you, it was a very helpful info. If the positive control and the control spike seems to be higher than expected. What do I need to do? The standard are in specification and R2 =1. Please advice and thank you
So interesting! Thank you! I am studying dopamine in humans and animals when active in Animal Assisted Therapy. What do you think about dopamine detected by ELISA kits from tear fluid before, during and after an intervention?
Thx for the video! Question: How does the antigen or BSA bind to the well? is it simply through hydrophobic interaction with the polyester plate? or is there any covalent interaction between antigen and plate?
