The use of Mobile Phase pH as a Method Development Tool 

Only the master can make tough concepts to very understandable way... u r my master, bhaskar sir

Beautifully explained importants of pka vs pH and their retention in RP-HPLC thank you Sir

Thank you for this, very helpful for method development. Cheers!

Simply and easy explanation, thank you for sharing such awesome content.

Grat work! carry on..❤

Excellent explanation, keep doing good work

nice information...its useful to pharma analysts

If sample contain more than 1 analyte I.e 2 or 3. How pka value and pH Mobil phase calculate??

Nice and simplest explain

Sir, if our compound having acidic and basic both, then how will manage the mobile phase pH.. please reply

Thanks Sir, it will be just opposite in normal phase column right? Please correct me if I'm wrong.

Thanks sir Nice explained sir

Could you tell me the app you use to draff, sir?

Good Sir & Thank u ...

Very good please go on about troubleshooting of HPLC

Very nice 👌

Sir please make video on HPLC column selecting

Really good content.

Superb Superb Superb Superb Superb Sir

PKa of my compound is 7.6. What should be the pH of my mobile phaase?

Compound remains in which state while retaining on column? Also plz make a vedio on behaviour of zwitterion in chromatography. How does it behave?

What a wonderful presentation it is, which I have never been gone through. how do we know the pka of new compound?

Sir, if both drug pka I'd 8.71 & 12.49 so, how can I select mobile phase.

Excellent sir,make more videos on usp general chapters

Thank you sir

Thank you❤

Nice sir..

But, acidic compounds retained in acidic pH and basic compounds retained in basic pH.

Sir what about neutal molecules?

Sir, what impact will be there on LC if Mobile phase's pka and Compound's Pka is same.?

I Want column chemistry

If pka is 17 , what should I have to do.

Dear sir, I have one doubt in analytical method validation. When the monograph method is used for validation in compound having more impurities and some of impurity (two or three) elutes same RT how to arrive RRF in this case further monograph mentioned RRF is 1. Please suggest the way forward 🙏

Why not Upload new Videos Sir

Why To Not Updated Any Videos

Valuable knowledge , thanks sir

Sir how to know compound acidc or base