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Using ImageJ to measure polyacrylamide gel images 

The BU Biochemistry Laboratory Manual
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Download ImageJ from the NIH here: imagej.nih.gov/ij/download.html
3:30 - How to download ImageJ
5:10 - Installation Windows users
8:30 - Installation for Mac users
11:48 - How to open your TIFF file on ImageJ and set rotation & dimensions
19:10 - Recognize your ladder
28:15 - Using the ROI manager and begin drawing and adding lines
40:01 - Saving and accessing your work
43:13 - Extract measurements and save as a .csv file
48:39 - Open file in Excel and generate your standard curve

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14 ноя 2020

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Комментарии : 9   
@LovelyLife365
@LovelyLife365 5 месяцев назад
Thanks for your easily described training video.
@chakkravarthyv8333
@chakkravarthyv8333 2 года назад
very nice video. Thanks a lot
@SalouaMABSOR
@SalouaMABSOR Год назад
so useful, thanks
@tarabridgetpierre573
@tarabridgetpierre573 3 месяца назад
Thank you so much for this video!! Quick question: Is the LDH going to be present on the standard curve?
@divyanshutiwari9916
@divyanshutiwari9916 2 года назад
The explanation is wonderful, if u can brief how can I identify a band of particular size on the gel?
@2women88
@2women88 2 года назад
what is the reason for changing from inches to meters, please?
@hananalenzi436
@hananalenzi436 3 года назад
Hello. Can you please give me your email because I need your help with image j. Thank you
@thebubiochemistrylaborator2333
@thebubiochemistrylaborator2333 3 года назад
Hello! You can e-mail us at biochemlabmanual@gmail.com
@livinghope8039
@livinghope8039 Год назад
long, complex and boring!
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