Hi! Thanks for this video! It was exactly what I needed to know. However, I wonder if you know if you have to have all the images open in image j before you press global? Or will it default to that scale in subsequent pictures you open, until you click to remove scale?
i have picture in which i have a sample with known length and there are humps in that sample. its a 2d picutre of sample. i want to cover its humps and want to put on xy-graph to measure its amplitude at various points.. i dont know how to do
Thank you very much for the tutorial. Can you make a tutorial on how to measure the length and diameter (for example images of tree root) of many images at once?
Hey all out there, great video to describe how to use the tool. But I have a task I have no solution for at the moment. For my analysis I want to measure the diameter of small spheres by setting three points on the edge of the spheres and then it should be possible draw a circle out of it. Does someone know, how and with which program this should be possible? Thanks in advance!
This is great. Very informative! My question is if you can set the scale for pictures with different pixels or they all have to be the exact same pixels from original file? I have different images with different pixels, and one image of a scale (index card) to use for measurement. Thanks in advance!
No the scale can be the same no matter what the file resolution is --- however, if you have different # of pixels, its likely that 1 pixel in one file does not correspond to the same physical measurement in the other file. However you can correct for that mathematically if you know what was going on in the two pictures. For example: if you took a picture of your scale at 400x400 resolution and then calculated the scale to be 1 pixel = 10 um. Then if all you do is change the number of pixels that are saved in the camera - so that your picture you want to measure is 1600x1600 pixels, then for that image the scale would be 1 pixel = 0.25 um. However if you change lenses or focal length or anything else, then your scale is no longer known.
Sir will you help me for some image processing, related to trichome (hair like structure on leaf) counting ? I am trying but not getting proper results.
Hey Dan, Thanks for this informative video, However I am still a bit confused for instance I need to measure the diameter of one Zone of inhibition in mm, how should I switch to mm ?
Sphericity seems like a 3D measurement, which we cannot do with this technique because we are reducing it down to 2D. You can calculate circularity from measurements made in ImageJ. There are several different mathematical ways to do that - you would have to choose the one you feel works best for you
You can use the "point" / multipoint tool --- next to the line tool is the angle tool and then next to that is the point tool. When you click to make a point and select "Measure (control-m), it will give you the X,Y coordinates of the point. You can click to make a lot of points and then measure them all at once and it will list them all.
@@danp444 Thank you very much. I have been using this command, it is taking a lot of time as I have on average 200 particles in a single image and need to process a large number of such images. So is there any shortcut to find coordinates? Regards.
I have never been able to understand what means for every record (i.e. n.3) Area, Min, Max and Mean. Is it perhaps connected to the bounding rectangle of the line?
Min, Max, and mean are related to the color (grayscale) of either the line or the box. The area is meaningless unless you have selected a 2D shape -- it is the area in pixels or whatever distance if you set in the scale
Hi Dan. thank you for this great video, very informative! for some reason its not working for me. I measure the diameter one by one and then I look at the results but they appear as one line only, maybe measuring the last diameter I drew. Have no idea how to solve this.... any suggestions? thanks!
hi! thank you for your help. However, each time i set a scale, once i open the window, it goes back to a random number. I am unable to keep the scale. what could be the problem and has anyone else faced the same issue?
Then I am not really sure...I don't think I've had that problem. The best way to mitigate that is just write down the pixels/mm and measure in pixels and do the math yourself I guess.
hello. I have a question.would you please help me?I want to count the number of grafts inside the plates with the Fiji (filament detector). how can i do that(can i have your email address i want to send photos of analysis)
