Western Blot

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( www.abnova.com ) - Western Blotting is an analytical technique used to detect specific proteins in a given sample. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide. The proteins are then transferred to a membrane where they are detected using antibodies specific to the target protein. More videos at Abnova www.abnova.com

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5 апр 2010

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Комментарии : 23

@XSirApocalypseX 14 лет назад

We learn those techniques (Microarray, Western Blot, Gel-electrophoresis, Southern and Northern Blot) in university theoretical and later practical and it's interesting to see it how it works.

@CR-uu1rr Год назад

Excellent and very clear. Detailed step by step. Amazing

@NoneroneousX5 12 лет назад

Wow. Real science one step at a time all lined up for us to see and made to look so easy. You had to have worked very hard too. thank you

@nicklinkzelda 14 лет назад

@XSirApocalypseX - that's how it works at my uni, too! We only briefly discussed Western blotting in a grad level course I took and now I'm actually doing Westerns in a lab! It is definitely much more different than what I imagined.

@reinaldofranqui1 13 лет назад

i have a test about this tomorrow i did it in the lab but this was waaay more comprehensive thanx!!!

@nicklinkzelda 14 лет назад

Karyotyping sounds amazing... though it is probably contamination prone considering the process. That's not something they even mentioned you could even do at my university. Thanks for sharing that bit of into.

@Lucuskane 12 лет назад

Best video I've watched on this site.

@Giaca89 12 лет назад

Exactly, you have to remember that you have many strips as the number of the samples you have added in wells for electrophoresys! You just have to remember which strips correspond to your samples ;)

@priboechaat 11 лет назад

This really helped me! Thank you very much! Great video!

@samsaras 14 лет назад

yes very superb professional handling skills. i'm gonna go into lab work.

@millie7995 11 лет назад

Would higher or lower percentage gels favour transfer to a membrane

@lovy42 11 лет назад

why haven't they put the chemiluminescent reagent directly on the membrane..?

@sonalverma905 9 лет назад

supperbbbbb ..!!!! nice explanation ..thanks a lot!! :-)

@khadidjabelgardi8624 4 года назад

who can help me I need principle and the pros and cons of this procedure

@kennetthkogo 11 лет назад

realy good

@salesmilton 13 лет назад

Is this test used for a single patient or multiple patients? If multiple, what is the maximum number of patients that can be tested.

@danielhdoro 13 лет назад

Seems easy in the video. Way different in a lab like mine.

@mouaynicholas 12 лет назад

lol we're doing this for our showcase project at cart. it's not that its hard, but you just gotta be careful on what your adding. for us, we have to start from scratch. making the blotting buffer, sample buffer, primary antibodies, secondary antibodies....yeesh. this is such a long project and a bit hard to understand.