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I'm currently working on a method where a pair of hydrophilic peaks elutes in less than 5 minutes, while another pair elutes about 15 minutes later in isocratic mode. When I use gradient methods (~50 to 70% ACN), the baseline drift increases significantly. What is the best way to run a gradient in these methods? Should I implement a steep gradient change in less than a minute or use a smoother gradient (over a longer period) ?
Thats very great,thanks for these useful points,could you please explain the thermostat and autosampler parts too?how can i understand the effeciency of these two parts
Sir can u please tell me... Which type of standards used for the Hplc of The sample of TSB broth inoculated with bacterial strains for growth with crude oil?
I’ve read that acetonitrile can have amine impurities and form deposits in pump components over time. Is there a procedure for flushing the system to remove/prevent this?
What about highly acidic or basic samples? Will that affect the C18 ligands of a column. I work with human serum and plasma that are treated with acid, precipitated and the supernate is injected so not the same as the mobile phase.
Thank you soo much explaining and making such informatve video.Looking for more such videos .Can you please do a video on peak purity and independent column compartment port switching in agilent in detail
