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# 1 Most Common Problem in HPLC: Air Bubble in the Pump 

Axion Labs & Training Institute
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The most common issue encountered in High-Performance Liquid Chromatography (HPLC) - an air bubble in the pump. Discover expert tips and techniques from Axion Labs to quickly and effectively resolve this problem.
Key Highlights:
Identifying Air Bubbles: Learn to recognize the symptoms of an air bubble in your HPLC pump, such as unusual pressure ripple and fluctuating UV signals​​.
Simple Fixing Method: We demonstrate a straightforward procedure to eliminate air bubbles, including opening the purge valve and adjusting the flow rate​​.
Verifying Solution Effectiveness: Understand how to check if the air bubble has been successfully removed by observing the pressure ripple and ensuring it falls within the acceptable range​​.
Software Tips: Gain insights into using diagnostic tools in HPLC software to monitor and confirm the pressure ripple status​​.
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Axion offers hands-on HPLC (High-performance liquid chromatography or high-pressure liquid chromatography) and GC (gas chromatography) training courses. Gain advanced HPLC & GC skills with our 5 day hands-on LC/GC bootcamp. If you can't make it to Chicago choose one of our online courses. After taking the LC/GC bootcamp further advance your skills by taking the advanced topics courses.
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17 окт 2022

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Комментарии : 4   
@bpopa
@bpopa Год назад
Love the pro tip of flushing the column completely by almost closing the valve all the way.
@365freekill
@365freekill Год назад
I followed your tip and it worked. Thank you so much!
@israrahmadsani4595
@israrahmadsani4595 Год назад
Kindly make a video on which properties of molecules require to develop acurate method development .. ( properties of molecules/drug vs which conditions or parameters should be select)
@israrahmadsani4595
@israrahmadsani4595 Год назад
Sir I want to develop a method for pelargonium sodiodes on hplc I tried methods available in research papers but that's are not working
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