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The second syringe already contains concentrated PRP. There is no reason to spin it again. However, if you wanted even more concentrated PRP you could spin it again and then separate the layers once more. But of course if you do this you will get a reduced yield because of platelet loss during the process.
great video! I used exactly your method and i am very happy with the results. I have one question though. If you withdraw the top 2/3 of plasma, you end up with a even more concetrate prp?
D2 is intended to be the distance to the buffy coat. So if you are using a gel tube (where the buffy coat will appear just above the gel) things get complicated. You'll have to break D2 down into its components and add them up. D2 has 2 components: (1) the distance from the top of the tube to the top of the column of blood, and (2) the distance from the top of the column of blood to the middle of the column of blood (before centrifugation). You can measure (1) directly on a filled gel tube. To get (2) you will have to measure the height of the column of blood in a filled gel tube and then divide by two. Then D2 = (1) + (2).
I think you may have misunderstood. We don't "add" any RBCs. We include the part of the buffy coat that is stained with RBCs because about half of the platelets are in that portion. Yes, this means we have some RBCs in the PRP. But without that portion our platelet yield would be much lower.
Thank you for this wonderful video. I do have a question. You mention removing ACD gel from the tubes before the second spin. Can we instead use tubes without ACD gel?
Most people use calcium. Either calcium gluconate or calcium chloride will work. We use 10%CaCl3 at a 1:20 ratio. You can also use thrombin or a host of other chemicals (platelets are very sensitive!) but we have no experience with these.
thank you for these videos. You mentioned in the videos that after preparing the plasma, you could cool it and administer it, or activate it and administer it. Does that mean that you do not necesarily have to activate it? thank you!
Whether you activate it or not will depend on your application. The platelets will be somewhat activated on injection by collagen in the tissue. If you want more activation in a localized area (tendon injections) then you would probably want to activate it before injection so that a fibrin clot will rapidly form and keep the platelets localized. If you want the PRP to spread and slowly activate (some cosmetic applications) then you would not want to activate it before injection.
I have heard such rumors, but I have never seen any evidence that it is so. Nor have I ever even seen a case report that might associate endotoxins with ACD-A tubes.
In Chinese society, we multiply platelet n the body by simply consuming the steamed essense of 2 frogs a day. Cancer patients who have low platelet cannot go for chemo... so 2 frogs a day for a week will boost blood platelet count dramatically to allow the patient to undergo chemo. Same for people who get Mosquito induce dengue, the virus chops down the blood platelet amounts and 2 frogs daily is the answer... The extraction method is called :Double Boiling: Check YT for the instructions. ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-3tRoAwxnrCo.html
VERY NICE VIDEO...THANK YOU!!! YOU SAY: 1. - WITHDRAW IT FROM TUBE, THEN INJECT IT IMMEDIATELY, OR 2. - COOL IT ND THEN WITHDRAW AND INJECT IT, OR, 3. - "ACTIVATE IT AND THEN INJECT IT, OR, 4. - JUST SAVE IT FOR LATER ON INJECTION. PLEASE...CAN YOU ANSWER THESE 3 QUESTIONS FOR ME: 1. WHAT DO YOU MEAN BY "ACTIVATE IT? 2. - HOW LONG CAN IT BE SAVED FOR BEFORE USING IT AGAIN? 3. - WHAT IS THE BEST TEMP TO COOL BEFORE WITHDRAWING AND INJECTING IT AGAIN, OR JUST TO KEEP IT COOL FOR LATER-USE-SAVING? THANK AGAIN FOR THIS AWESOME, VERY INFORMATIVE VIDEO...CHEERS!!!
"Activate" means to add an agent such as calcium or thrombin that will cause the platelets to release their alpha granules. How long can you save PRP? Unknown. It will gradually deteriorate over time so there is no clear cutoff point. Perhaps less than 24 hours. Cooling it right before injection sensitizes the platelets. That's what you want.
